Single Cell Sequencing Technology and Its Application in Hair Follicle Development

doi:10.13560/j.cnki.biotech.bull.1985.2020-1491

Abstract

Abstract:

Hair follicle is a kind of micro-organ with unique structure and periodic growth,its morphogenesis begins in the embryonic stage and is induced by a series of interactions among epidermis epithelium,interstitial dermis and special derivatives. After birth,individual hair follicles begin to cycle periodically,including growth period,retrogression period and resting period. Single-cell RNA sequence（scRNA-seq）is a new sequencing method,mainly through the preparation of single cell suspension or cell groups and using the next generation sequencing to identify the gene expression information of the single cell. It is mainly used to analyze the differences of genetic and gene expression levels among cells,to better understand the specific role of a single cell in the microenvironment. The regulatory relationship between complex and rare cell populations and genes may be revealed via scRNA-seq,and the developmental trajectories of different cell lineages can be traced. This paper describes the scRNA-seq and its application in the regulation of hair follicle development,aiming to provide theoretical reference for revealing the molecular regulation mechanism of hair follicle development.

Key words: single-cell RNA sequencing, single cell isolation technique, hair follicle development

YE Na, ZHANG Xiao-lan, BAO Peng-jia, WANG Xing-dong, YAN Ping, PAN He-ping. Single Cell Sequencing Technology and Its Application in Hair Follicle Development[J]. Biotechnology Bulletin, 2021, 37(10): 245-256.

Figures/Tables 2

References 86

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方法 Method	cDNA合成 cDNA synthesis	扩增方法 Amplification method	捕获细胞量 Quantity of captured cells	cDNA覆盖 cDNA cover	Barcode	UMI	参考文献Reference
Tang2009	ployA尾	PCR	~10	3'端偏移的全长	No	No	[23]
Quartz-seq	ployA尾	PCR	1 000-10 000	3'端偏移的全长	No	No	[76]
SUPeR-seq	ployA尾	PCR	~10	全长	Yes	No	[67]
MATQ-seq	ployA尾	PCR	10-100	全长	Yes	Yes	[68]
STRT-seq	5'端模板转换	PCR	100-1 000	5'末端	Yes	Yes	[37]
Smart-seq	5'端模板转换	PCR	100-1 000	3'偏倚的全长	No	No	[41]
10xGenomics	5'端模板转换	PCR	1 000-10 000	3'末端	Yes	Yes	[44]
Seq-well	5'端模板转换	PCR	100-1 000	3'末端	Yes	Yes	[46]
Microwell-seq	5'端模板转换	PCR	100-10 000	全长	Yes	Yes	[45]
Drop-seq	5'端模板转换	PCR	1 000-10 000	3'末端	Yes	Yes	[43]
CEL-seq	IVT	IVT	100-1 000	3'末端	Yes	Yes	[73]
MARS-seq	IVT	IVT	1 000-5 000	3'末端	Yes	Yes	[27]
inDrops	IVT	IVT	1 000-10 000	3'末端	Yes	Yes	[42]

方法 Method	cDNA合成 cDNA synthesis	扩增方法 Amplification method	捕获细胞量 Quantity of captured cells	cDNA覆盖 cDNA cover	Barcode	UMI	参考文献Reference
Tang2009	ployA尾	PCR	~10	3'端偏移的全长	No	No	[23]
Quartz-seq	ployA尾	PCR	1 000-10 000	3'端偏移的全长	No	No	[76]
SUPeR-seq	ployA尾	PCR	~10	全长	Yes	No	[67]
MATQ-seq	ployA尾	PCR	10-100	全长	Yes	Yes	[68]
STRT-seq	5'端模板转换	PCR	100-1 000	5'末端	Yes	Yes	[37]
Smart-seq	5'端模板转换	PCR	100-1 000	3'偏倚的全长	No	No	[41]
10xGenomics	5'端模板转换	PCR	1 000-10 000	3'末端	Yes	Yes	[44]
Seq-well	5'端模板转换	PCR	100-1 000	3'末端	Yes	Yes	[46]
Microwell-seq	5'端模板转换	PCR	100-10 000	全长	Yes	Yes	[45]
Drop-seq	5'端模板转换	PCR	1 000-10 000	3'末端	Yes	Yes	[43]
CEL-seq	IVT	IVT	100-1 000	3'末端	Yes	Yes	[73]
MARS-seq	IVT	IVT	1 000-5 000	3'末端	Yes	Yes	[27]
inDrops	IVT	IVT	1 000-10 000	3'末端	Yes	Yes	[42]