Study on Urine Metabolomics in Rats of Different Ages

doi:10.13560/j.cnki.biotech.bull.1985.2021-0331

Abstract

Abstract:

This work aims to explore the age-related changes of urine metabolites in healthy rats during growth,development and aging. Urine samples were collected at 3,5,7,9,12,56,and 111 weeks after birth,and the GC/TOF-MS platform was employed for metabolomics detection. The results showed that all urine samples appeared in-group clustering and between-group separation in principal component analysis and partial least square discriminant analysis. Amino acids,organic acids,and carbohydrates were the dominant metabolite types in the rat urine samples. The levels of urine metabolites such as 4-hydroxyproline,L-lysine,and uric acid gradually decreased with age. D-ribose,aconitic acid,and fumaric acid increased in old age,while nicotinic acid and 5-hydroxyindole decreased in old age. The above results revealed that age affected urine metabolism in rats,and a variety of urine metabolites appeared age-related changes during growth,development and aging.

Key words: growth, development, aging, metabolomics, urine metabolites

YANG Yu-ping, ZHANG Xia, WANG Chong-chong, WANG Xiao-yan. Study on Urine Metabolomics in Rats of Different Ages[J]. Biotechnology Bulletin, 2022, 38(2): 166-172.

Figures/Tables 4

Fig.1 Schematic diagram of rat urine sampling time points and age segments

Fig.2 Scatter plots of rat urine metabolism profile A：Principal component analysis（PCA）. B：Partial least squares discriminant analysis（PLS-DA）

Fig.3 Relative abundance of metabolite types in rat urine with different age segments

Fig.4 Fold change heatmaps of all urine metabolites The fold change values of >2 are represented by 2. *P < 0.05,**P < 0.01,***P < 0.001

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