Effects of Shading on the Active Component 10-DAB and Mineral Nutrient Accumulation of Taxus madia × T. Yunnanensis ‘Yunman’

doi:10.13560/j.cnki.biotech.bull.1985.2022-0353

Abstract

Abstract:

Light is a necessary environment factor for plant growth. Revealing the effects of different shading treatments on the active component 10-deacetylbaccatine III（10-DAB），mineral nutrient accumulation and photosynthesis of Taxus madia × T.Yunnanensis ‘Yunman’（YM）seedlings would provide a scientific basis for the comprehensive utilization of YM forest and the construction of forest-grass composite ecosystems. 2-year-old yews of YM seedlings were used as materials，and 5 light intensity conditions（full light，30% shading，50% shading，75% shading，and 95% shading）were set to clarify the response strategy of YM to different light environments. The results showed that shading had a significant effect on the accumulation of 10-DAB in each growth period of YM seedlings（P<0.05）. The yield of 10-DAB showed an upward trend from April to October and reached a maximum in October. With the enhancement of shade，the accumulation of mineral elements in each part showed a trend of increasing first and then decreasing. Among them，N and P were the highest under 75% shading treatment，and the contents of mineral nutrition in each part were in the order of leaf > root > stem，K was the highest under 50% shading treatment，and its content was in the order of leaf > stem > root. The photosynthetic pigment content of YM increased significantly under shading（P<0.05），and increased first and then decreased with the increase of shading. Under 75% shading，the photosynthetic pigment content was the highest. The changes in net photosynthetic rate（P_n），stomatal conductance（G_s），and transpiration rate（T_r）were consistent with the photosynthetic pigment content. The intercellular CO₂ concentration（C_i）decreased first and then increased，and the indexes（except C_i）were the lowest under 95% shading. In summary，heavy shading inhibited the photosynthesis of YM. Shading intensity can effectively improve 10-DAB yield and mineral nutrient accumulation of YM seedlings（branches and leaves），and 50%-75% shading is suitable for YM seedlings cultivation.

Key words: shading, Taxus madia × T. Yunnanensis ‘Yunman’, 10-DAB accumulation, nutrient accumulation, photosynthetic characteristics

WANG Gang, LUO Jian-xun, PU Shang-rao, LI Ya-ping, WANG Gang, SUN Zhi-peng. Effects of Shading on the Active Component 10-DAB and Mineral Nutrient Accumulation of Taxus madia × T. Yunnanensis ‘Yunman’[J]. Biotechnology Bulletin, 2022, 38(11): 175-184.

Figures/Tables 9

Fig. 1 10-DAB content and branch and leaf biomasses of YM（Taxus madia × T. Yunnanensis ‘Yunman’）seedlings in different shading treatments Different lowercase letters indicate significant differences among shading treatments（P<0.05）. The same below

Fig. 2 Effects of shading on the 10-DAB accumulation of YM seedlings

Fig. 3 Effects of shading treatments on the N，P and K contents in the roots，stems and leaves of YM seedlings

Fig. 4 Effects of shading treatments on the N，P and K accumulations of YM seedlings

Fig. 5 Correlation analysis of N，P and K contents and th-eir stoichiometric ratios in the roots（a），stems（b）and leaves（c）of young YM seedlings * indicate P<0.05, ** indicate P<0.01

Fig. 6 Regression analysis of N and P in the root（a），stem（b），leaf（c）of YM seedlings

Table 1 Effects of shading treatments on the chlorophyll and carotenoid content in the leaves of YM seedlings

遮荫处理Shading treatment	叶绿素a Chl a/（mg·g^-1）	叶绿素b Chl b/（mg·g^-1）	总叶绿素Total Chl/（mg·g^-1）	类胡萝卜素Carotenoids/（mg·g^-1）
CK	0.818±0.047b	0.627±0.034b	1.433±0.065b	0.563±0.029b
30%	0.957±0.059b	0.823±0.120a	1.787±0.237a	0.712±0.084a
50%	1.372±0.120a	0.835±0.129a	2.206±0.349a	0.841±0.149a
75%	1.532±0.112a	0.853±0.061a	2.315±0.231a	0.865±0.053a
95%	0.728±0.041b	0.535±0.037b	1.262±0.071b	0.496±0.053b

Table 2 Effects of shading treatments on the relevant photosynthetic parameters of YM seedlings

遮荫处理 Shading treatment	净光合速率 P_n/ （μmol·m^-2·s^-1）	气孔导度 G_s/ （mol·m^-2·s^-1）	蒸腾速率T_r/ （mmol·m^-2·s^-1）	胞间CO₂浓度C_i/ （μmol·mol^-1）
CK	2.041±0.277b	0.071±0.077b	117.641±8.039b	6.496±0.202b
30%	2.971±0.093b	0.100±0.011ab	125.86±10.850b	2.558±0.073c
50%	4.509±0.245a	0.090±0.009ab	340.43±18.821a	0.853±0.078d
75%	5.298±0.806a	0.158±0.006a	439.322±8.357a	0.575±0.053d
95%	1.351±0.111b	0.057±0.017b	58.547±2.089c	8.686±0.113a

Fig. 7 Light response curve in the leaves of YM under diff-erent shading treatments

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