Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (4): 10-23.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0985

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Redox Partner Engineering: A Solution to the Low Catalytic Efficiency of P450s

ZHANG Yan-feng(), YE Li-dan(), YU Hong-wei()   

  1. College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027
  • Received:2022-08-13 Online:2023-04-26 Published:2023-05-16

Abstract:

Cytochrome P450 enzymes(CYPs or P450s)can insert one atom of O2 into organic substrates and reduce another atom to water. As P450s are widely involved in various anabolic and catabolic processes, they have always been a focus of biotechnology. Oxidation of substrates in the catalytic cycle relies on the transfer of electrons from redox partners to the heme iron, suggesting electron transfer as a rate-limiting step in the catalytic process of P450s. Optimization of protein-protein interactions to improve the electron transfer efficiency of the P450 system, known as “redox partner engineering”, is one of the important means to engineer P450s, and has achieved fruitful progress. This paper will focus on the progress in the replacement and assembly of redox partners, fusion of P450 enzymes with redox partners, and interface modification between P450 enzymes and redox partners, aiming to provide some guidance for future work in this area.

Key words: cytochrome P450 enzymes, redox partners, electron transfer, component replacement, fusion enzymes