Mechanism of Melatonin Seed Priming in Improving Salt Tolerance of Capsicum annuum

doi:10.13560/j.cnki.biotech.bull.1985.2022-1253

Abstract

Abstract:

Melatonin can enhance plant salt tolerance, but its effect and mechanism of improving plant salt tolerance through seed priming are rarely reported. In order to investigate the effects of melatonin（MT）seed priming on the growth and physiological characteristics of pepper seedlings under salt stress, its influences on the growth, photosynthetic characteristics, antioxidant metabolism and osmotic regulation of pepper seedlings under salt stress were studied using ‘Maoshu 360’ as material. The results showed that MT seed priming significantly alleviated the adverse effects of salt stress on the growth of pepper plants. Compared with seed without seed priming treatment, seed priming treatments with different concentrations of melatonin significantly improved the growth of pepper seedlings under salt stress, specifically, these indexes plant root length, above ground height, stem fresh weight, leaf fresh weight, root fresh weight, FV'/Fm', Qp, NPQ, Fo, FV/Fm and chlorophyll content increased. Physiological analysis showed that the contents of malondialdehyde, hydrogen peroxide and superoxide anion in the leaves of pepper under salt stress significantly reduced by seed priming with different concentrations of melatonin. The contents of soluble sugar, soluble protein, proline, AsA and DHA in the leaves significantly increased. The activities of POD, SOD, APX and GR significantly increased. In addition, melatonin seed priming treatment significantly increased the content of K⁺ in the leaves, stems and roots, and decreased the content of Na⁺ and Na⁺/ K⁺in the leaves of seedlings under salt stress. The above results showed that 100 μmol/L melatonin seed priming treatment had the best effect, which significantly improved the tolerance to soil salt, reduced the absorption of Na⁺ by plants, and alleviated the damage of salt stress to plants.

Key words: salt stress, Capsicum annuum, melatonin, leaf blade, anti-oxidation, regulation of osmosis

WEI Xi-ya, QIN Zhong-wei, LIANG La-mei, LIN Xin-qi, LI Ying-zhi. Mechanism of Melatonin Seed Priming in Improving Salt Tolerance of Capsicum annuum[J]. Biotechnology Bulletin, 2023, 39(7): 160-172.

Figures/Tables 10

Fig. 1 Effects of seed priming with melatonin（MT）at different concentration on the root length（A）, above ground height（B）and root shoot ratio（C）of C. annuum under salt stress The above data are mean ± standard deviation（n=3）. Different letters represent significant differences（P<0.05）. The same below

Fig. 2 Effects of melatonin at different concentration on the plant fresh weight（A）, leaf fresh weight（B）, stem fresh weight（C）and root fresh weight（D）of C. annuum under salt stress

Fig. 3 Effects of melatonin at different concentration on the plant dry weight（A）, leaf dry weight（B）, stem dry weight（C）and root dry weight（D）of C. annuum under salt stress

Fig. 4 Chlorophyll fluorescence parameter Fv'/Fm'（A）, Qp（B）, NPQ（C）, Fo（D）, Fv/F0（E）, Fv/Fm（F）and SPAD value（G）in the leaves of C. annuum affected by melatonin at different concentration

Fig. 5 Effects of melatonin at different concentration on the contents of MDA（A）, hydrogen peroxide（B）and superoxide anion（C）in the leaves ofC. annuum under salt stress

Fig. 6 Effects of melatonin at different concentrations on the contents of soluble sugar（A）, soluble protein（B）and proline（C）in the leaves of C. annuum under salt stress

Fig. 7 Effects of melatonin at different concentrations on the POD（A）, CAT（B）and SOD（C）activities in the leaves of C. annuum under salt stress

Fig. 8 Effects of melatonin at different concentration on the APX（A）and GR（B）activities in the leaves of C. annuum under salt stress

Fig. 9 Effects of melatonin different concentrations on the AsA（A）, DHA（B）and AsA/DHA contents（C）in the leaves of C. annuum under salt stress

Fig. 10 Melatonin at different concentrations affecting the contents and proportions of Na+（A）, K+（B）and Na+/K+（C）in the leaves, stems and roots of C. annuum under salt stress

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