Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (7): 67-79.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1426
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LI Yu-zhen1(), MEI Tian-xiu1, LI Zhi-wen1, WANG Qi1, LI Jun2, ZOU Yue2, ZHAO Xin-qing1()
Received:
2022-11-18
Online:
2023-07-26
Published:
2023-08-17
Contact:
ZHAO Xin-qing
E-mail:Lyz781601267@sjtu.edu.cn;xqzhao@sjtu.edu.cn
LI Yu-zhen, MEI Tian-xiu, LI Zhi-wen, WANG Qi, LI Jun, ZOU Yue, ZHAO Xin-qing. Advances in Genomic Studies and Metabolic Engineering of Red Yeasts[J]. Biotechnology Bulletin, 2023, 39(7): 67-79.
红酵母种Red yeast species | 活性成分及用途Active ingredients and uses | 参考文献Reference |
---|---|---|
黏红酵母 R. glutinis | 红色素、类胡萝卜素、多糖、辅酶Q10、橄榄的软化、处理工业污水 | [ |
胶红酵母 R. mucilaginosa | 氨基酸、酶、番茄红素、β-胡萝卜素、虾青素、多种脂肪酸、多糖、化妆品成分、饲料添加剂 | [ |
圆红酵母 R. toruloides | 类胡萝卜素、虾青素、油脂、倍半萜、脂肪酸酯、脂肪醇、化妆品成分 | [ |
克氏红酵母 R. kratochvilovae | 脂类、外糖脂、β-葡聚糖、类胡萝卜素 | [ |
Table 1 Active ingredients and uses of red yeast
红酵母种Red yeast species | 活性成分及用途Active ingredients and uses | 参考文献Reference |
---|---|---|
黏红酵母 R. glutinis | 红色素、类胡萝卜素、多糖、辅酶Q10、橄榄的软化、处理工业污水 | [ |
胶红酵母 R. mucilaginosa | 氨基酸、酶、番茄红素、β-胡萝卜素、虾青素、多种脂肪酸、多糖、化妆品成分、饲料添加剂 | [ |
圆红酵母 R. toruloides | 类胡萝卜素、虾青素、油脂、倍半萜、脂肪酸酯、脂肪醇、化妆品成分 | [ |
克氏红酵母 R. kratochvilovae | 脂类、外糖脂、β-葡聚糖、类胡萝卜素 | [ |
物种名称Organism name | 菌种Strain | BioProject | 测序水平Level | 大小Size/Mb | Scaffolds | 编码序列CDS |
---|---|---|---|---|---|---|
R. mucilaginosa | CYJ03 | PRJNA506114 | Contig | 19.037 2 | 88 | 6301[ |
R. mucilaginosa | KR | PRJNA435582 | Scaffold | 20.066 2 | 359 | 7059 |
R. toruloides | CGMCC 2.1609 | PRJNA297267 | Scaffold | 33.389 7 | 365 | 9820[ |
R. toruloides | CBS 14 | PRJEB40807 | Contig | 20.534 9 | 3 | 9464[ |
R. kratochvilovae | CBS 7436 | PRJNA643234 | Scaffold | 21.685 7 | 357 | - |
R. kratochvilovae | VS II C KN 5 | PRJNA644637 | Scaffold | 21.857 6 | 376 | - |
R. glutinis | ZHK | PRJNA605146 | Scaffold | 22.325 5 | 30 | 6774[ |
R. glutinis | ATCC 204091 | PRJNA59971 | Scaffold | 20.478 9 | 29[ | 3359[ |
R. babjevae | CBS 7808 | PRJEB48745 | Contig | 21.862 4 | 3[ | 7591[ |
R. babjevae | DBVPG 8058 | PRJEB48745 | Contig | 21.522 1 | 1[ | 7481[ |
R. paludigena | CM33 | PRJNA491831 | Scaffold | 20.655 4 | 78 | - |
R. paludigena | P4R5 | PRJNA732286 | Scaffold | 20.967 9 | 277 | - |
Table 2 Gene assembly and annotation data for selected species of the genus Rhodotorula
物种名称Organism name | 菌种Strain | BioProject | 测序水平Level | 大小Size/Mb | Scaffolds | 编码序列CDS |
---|---|---|---|---|---|---|
R. mucilaginosa | CYJ03 | PRJNA506114 | Contig | 19.037 2 | 88 | 6301[ |
R. mucilaginosa | KR | PRJNA435582 | Scaffold | 20.066 2 | 359 | 7059 |
R. toruloides | CGMCC 2.1609 | PRJNA297267 | Scaffold | 33.389 7 | 365 | 9820[ |
R. toruloides | CBS 14 | PRJEB40807 | Contig | 20.534 9 | 3 | 9464[ |
R. kratochvilovae | CBS 7436 | PRJNA643234 | Scaffold | 21.685 7 | 357 | - |
R. kratochvilovae | VS II C KN 5 | PRJNA644637 | Scaffold | 21.857 6 | 376 | - |
R. glutinis | ZHK | PRJNA605146 | Scaffold | 22.325 5 | 30 | 6774[ |
R. glutinis | ATCC 204091 | PRJNA59971 | Scaffold | 20.478 9 | 29[ | 3359[ |
R. babjevae | CBS 7808 | PRJEB48745 | Contig | 21.862 4 | 3[ | 7591[ |
R. babjevae | DBVPG 8058 | PRJEB48745 | Contig | 21.522 1 | 1[ | 7481[ |
R. paludigena | CM33 | PRJNA491831 | Scaffold | 20.655 4 | 78 | - |
R. paludigena | P4R5 | PRJNA732286 | Scaffold | 20.967 9 | 277 | - |
Fig. 2 Research and modification of red yeast SCO: Single cell oil. Genome editing of red yeast includes insertion and deletion of genes using the CRISPR-Cas system and Agrobacterium tumefaciens-mediated genetic transformation, followed by test and analysis at the level of phenotype, mRNA translation, protein expression, etc. The target strain can then be used for industrial production of lipids, carotenoids, and other substances
目标性状 Targeted trait | 代谢工程策略 Metabolic engineering strategies | 生物来源 Source organism | 结果 Outcomes | 参考文献 Reference |
---|---|---|---|---|
Limonene production | Overexpressed the limonene synthase, neryl pyrophosphate synthase/geranyl pyrophosphate synthase and the native hydroxy-methyl-glutaryl-CoA reductase | R. toruloides NP11 | Produced a maximum limonene titer of 393.5 mg/L | [ |
CoQ10, sterols, and phytoene accumulation | a T-DNA insertion in the CAR1 gene coding for phytoene desaturase | R. mucilaginosa C2.5t1 | Significant decreases in cell density and fatty acids content, higher productions of phytoene, CoQ10, and sterols | [ |
Fatty alcohol production | Two overexpression targets(ACL1 and ACC1)and two deletion targets(the acyltransferases DGA1 and LRO1) | R. toruloides IFO0880 | 1.8 to 4.4-fold increases to the fatty alcohol titer in culture tubes | [ |
Fatty acid ethyl esters production | Introduced various wax ester synthase genes from different sources, mutated bifunctional enzyme to abolish the DGAT activity | R. toruloides Δku70 | Improved the FAEEs titer to 1.02 g/L | [ |
Fatty acid production | Overexpressed acetyl coenzyme A carboxylase(ACC1 carboxylase)gene and repressed 3-hydroxy 3-methylglutaryl reductase(HMG-CoA reductase) | R. mucilaginosa Y-1 | Acombination of both inhibitor and inducer resulted in a 57% increase in lipid concentration | [ |
Carotenoid biosynthesis | Constructed vectors pZPK-CRT-1 and pZPK-CRT-2 with strong promoters PGPD and TADH2 | R. toruloides NP11 | The intracellular carotenoid content of the transformants increased by 30% | [ |
Table 3 Summary of metabolic engineering modification of red yeast
目标性状 Targeted trait | 代谢工程策略 Metabolic engineering strategies | 生物来源 Source organism | 结果 Outcomes | 参考文献 Reference |
---|---|---|---|---|
Limonene production | Overexpressed the limonene synthase, neryl pyrophosphate synthase/geranyl pyrophosphate synthase and the native hydroxy-methyl-glutaryl-CoA reductase | R. toruloides NP11 | Produced a maximum limonene titer of 393.5 mg/L | [ |
CoQ10, sterols, and phytoene accumulation | a T-DNA insertion in the CAR1 gene coding for phytoene desaturase | R. mucilaginosa C2.5t1 | Significant decreases in cell density and fatty acids content, higher productions of phytoene, CoQ10, and sterols | [ |
Fatty alcohol production | Two overexpression targets(ACL1 and ACC1)and two deletion targets(the acyltransferases DGA1 and LRO1) | R. toruloides IFO0880 | 1.8 to 4.4-fold increases to the fatty alcohol titer in culture tubes | [ |
Fatty acid ethyl esters production | Introduced various wax ester synthase genes from different sources, mutated bifunctional enzyme to abolish the DGAT activity | R. toruloides Δku70 | Improved the FAEEs titer to 1.02 g/L | [ |
Fatty acid production | Overexpressed acetyl coenzyme A carboxylase(ACC1 carboxylase)gene and repressed 3-hydroxy 3-methylglutaryl reductase(HMG-CoA reductase) | R. mucilaginosa Y-1 | Acombination of both inhibitor and inducer resulted in a 57% increase in lipid concentration | [ |
Carotenoid biosynthesis | Constructed vectors pZPK-CRT-1 and pZPK-CRT-2 with strong promoters PGPD and TADH2 | R. toruloides NP11 | The intracellular carotenoid content of the transformants increased by 30% | [ |
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