Preparation of Immobilized Lipase for the Solvent-free Synthesis of Cinnamyl Acetate

doi:10.13560/j.cnki.biotech.bull.1985.2023-0028

Abstract

Abstract:

Hydrophobically ordered mesoporous SiO₂（OMS）carrier was prepared by template method combined with octyl surface modification, and immobilized lipase was prepared on this basis（CSL@OMS-C₈）, which was successfully applied to the solvent-free enzymatic preparation of cinnamyl acetate. Scanning electron microscope（SEM）, transmission electron microscopy（TEM）, nitrogen gas adsorption analyzer, Fourier transform infrared（FTIR）and contact angle goniometer were employed to characterize the carriers and immobilized enzyme. The results showed that OMS has ordered mesoporous morphology with a specific surface area of 149.9 m²/g and corresponding average pore diameter of 15 nm. The hydrophobicity of the material was greatly improved after hydrophobic modification with the contact angle increased from 20° to 120°. The optimal reaction conditions were obtained by optimizing the reaction conditions of enzymatic transesterification: reaction temperature was 50℃, molar ratio of cinnamyl alcohol to vinyl acetate was 1∶5, catalyst addition was 2 g/L, reaction time was 2 h, and the conversion rate reached 96.6%. Besides, the CSL@OMS-C₈ maintained 80% conversion for cinnamyl alcohol after five-cycle reuse.

Key words: immobilized lipase, solvent-free, flavor ester, cinnamyl acetate, transesterification

CHEN Jin-hang, ZHANG Yi, ZHANG Jun-tao, WEI Ben-mei, WANG Hong-xun, ZHENG Ming-ming. Preparation of Immobilized Lipase for the Solvent-free Synthesis of Cinnamyl Acetate[J]. Biotechnology Bulletin, 2023, 39(9): 97-104.

Figures/Tables 6

Fig. 1 Lipase screening Different letters indicate significant differences among treatments（P<0.05）. The same below

Fig. 2 Microscopic observation results of material OMS a: SEM results. b: TEM results. c: N2 adsorption-desorption of OMS, OMS-C8 and CSL@OMS-C8. d: Pore size distribution curves of OMS, OMS-C8 and CSL@OMS-C8

Fig. 3 FT-IR spectra of the free lipase CSL, OMS, OMS-C8 and CSL@OMS-C8

Fig. 4 Hydrophobic measurement for OMS（A）and OMS-C8（B）

Fig. 5 Effects of different reaction conditions on the synthesis of cinnamyl acetate and comparison of immobilized and free enzymes under optimal conditions a: Effects of substrate molar ratio on the synthesis of cinnamyl acetate; b: effects of temperature on the synthesis of cinnamyl acetate; c: effects of enzyme loading on the synthesis of cinnamyl acetate; d: comparison of free enzyme and immobilized lipase under the optimal conditions

Fig. 6 Reusability of CSL@OMS-C8

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