Identification of the Kiwifruit BBX Gene Family and Analysis of Their Transcriptional Characteristics

doi:10.13560/j.cnki.biotech.bull.1985.2023-0812

Abstract

Abstract:

【Objective】 BBX（B-Box）, as one of the subfamilies of zinc finger transcription factors, is widely involved in the plant growth and development process. To reveal the genetic characteristics and potential functions of the kiwifruit BBX family.【Method】 The kiwifruit BBX gene family was identified at the whole genome level. And the protein physicochemical properties, subcellular localizations, systemic developmental characteristics, conserved protein domains, and homologous characteristics were analyzed. Meanwhile, the expression patterns in different tissues and different storage treatments were analyzed.【Result】 A total of 48 AcBBX genes were unevenly distributed across 22 chromosomes. The amino acid length of AcBBXs was 126-515 aa, protein molecular weight was 14 172.09-57 905.84 Da, and four subcellular localizations were predicted. There were 0 to 4 introns in AcBBX family. AcBBX promoters contained various cis-acting elements involved in light responsiveness, plant hormone regulatory, biotic and abiotic stress regulatory, growth and development regulatory. Combined the evolutionary tree with conserved domains, they could be clustered into five groups, and gene tandem duplication and fragment replication were the main reasons for the expansion of family members. The expressions of AcBBXs varied in different organs of kiwifruit. Seven AcBBX members had higher expressions in the mature fruits compared to other parts, 3 members having higher expressions in the young fruits, 7 members having higher expressions in the leaves, and 3 members having higher expressions in the flowers. During the post-harvest storage of fruits, 3 members were upregulated by exogenous hormones and higher than other processing groups, while 6 members were upregulated after one week of storage at room temperature and higher than other processing groups. During 4℃ storage of fruits, 10 members were upregulated, while 5 members were downregulated.【Conclusion】 This study reveals the transcriptional characteristics of different members of the kiwifruit AcBBX family during its growth, development, and post-harvest storages, laying the foundation for subsequent functional verification of the AcBBX gene.

Key words: kiwifruit, BBX transcription factor, whole genome identification, different tissues, fruit storage, transcription analysis

LU Yu-dan, LIU Xiao-chi, FENG Xin, CHEN Gui-xin, CHEN Yi-ting. Identification of the Kiwifruit BBX Gene Family and Analysis of Their Transcriptional Characteristics[J]. Biotechnology Bulletin, 2024, 40(2): 172-182.

Figures/Tables 6

Fig. 1 Phylogenetic analysis of the BBX genes between kiwifruit and Arabidopsis I-V: Five groups of AcBBX. Kiwifruit AcBBXs are in red, Arabidopsis AtBBXs are in black

Fig. 2 Motif（A）, conserved domains（B）and gene structures（C）of AcBBX gene family

Fig. 3 Analysis of cis-acting elements in AcBBX genes

Fig. 4 Intraspecific collinearity of AcBBX genes and their chromosome density and locations ①: Gene chromosome locations. ②: Gene chromosome density heatmap. ③: Gene chromosome density line map. ④: Intra-species collinearity map

Fig. 5 Expression patterns of the AcBBX genes in different tissues of kiwifruit

Fig. 6 Expression patterns of the AcBBX genes under different storage treatments CK: Mature fruits（160 d post flowering）; RT_1W: mature fruits stored at 25℃ for 1 week; ABA_1W: mature fruits soaked with 50 mg/L ABA for 2 min and then stored at 25℃ for 1 weeks; Four_1W: mature fruits stored at 4℃ for 1 week; Four_2W: mature fruits stored at 4℃ for 2 weeks; Four_3W: mature fruits stored at 4℃ for 3 weeks

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