Synergistic Remediation of Multiple Pollutants in Agricultural Environment by Microorganisms and Biochar

doi:10.13560/j.cnki.biotech.bull.1985.2024-0691

Abstract

Abstract:

Agricultural environmental pollution has posed a great challenge to crop production in China, and it is urgent to take effective control of it. Microbe-based environmental remediation technologies, without secondary pollution, are characterized by high efficiency and low operation cost, which has attracted increasing interest. Moreover, the combination of functional microbe and biochar can further improve the efficiency in eliminating environmental pollutants, and positive results have been achieved. In this context, we first summarized the role of functional microorganisms in the remediation of environmental pollutants and the current application status of biochar-loaded microorganisms in the environmental remediation. We then discussed the advantages and challenges of biochar-loaded microorganism technology in the remediation efficiency and resource utilization. Based on the development of current technology, we proposed the direction of further research on the biochar-loaded microorganism remediation technology, providing a basis for in-depth understanding of the effectiveness and safety of relevant techniques.

Key words: soil heavy metal, pesticide residues, water eutrophication, antibiotic residues

LI Chong, YANG Ya-nan, WANG Cui-xia, ZHENG Hai-xin. Synergistic Remediation of Multiple Pollutants in Agricultural Environment by Microorganisms and Biochar[J]. Biotechnology Bulletin, 2024, 40(10): 86-97.

Figures/Tables 5

References 76

[1]	Zhang HY, Yuan XZ, Xiong T, et al. Bioremediation of co-contaminated soil with heavy metals and pesticides: influence factors, mechanisms and evaluation methods[J]. Chem Eng J, 2020, 398: 125657.
[2]	环境保护部, 国土资源部. 全国土壤污染状况调查公报[R]. 北京: 中国政府网, 2014.
	Ministry of Environmental Protection, Ministry of Natural Resources. National Soil Pollution Survey Report[R]. Beijing: Chinese government network, 2014.
[3]	Chen YG, He XLS, Huang JH, et al. Impacts of heavy metals and medicinal crops on ecological systems, environmental pollution, cultivation, and production processes in China[J]. Ecotoxicol Environ Saf, 2021, 219: 112336.
[4]	广东省生态环境厅. 2023年广东省生态环境状况公报[R]. 广州: 广东省生态环境厅, 2024.
	Ecological environment of Guangdong province. Bulletin on the Ecological Environment Status of Guangdong Province in 2023[R]. Guangzhou: Ecological environment of Guangdong province, 2024.
[5]	Gadd GM. Metals, minerals and microbes: geomicrobiology and bioremediation[J]. Microbiology, 2010, 156(Pt 3): 609-643.
[6]	Gałązka A, Jankiewicz U. Endocrine disrupting compounds(nonylphenol and bisphenol A)-sources, harmfulness and laccase-assisted degradation in the aquatic environment[J]. Microorganisms, 2022, 10(11): 2236.
[7]	Zhan PR, Liu W. Use of fluidized bed biofilter and immobilized Rhodopseudomonas palustris for ammonia removal and fish health maintenance in a recirculation aquaculture system[J]. Aquac Res, 2013, 44(3): 327-334.
[8]	Liao XB, Zou RS, Li BX, et al. Biodegradation of chlortetracycline by acclimated microbiota[J]. Process Saf Environ Prot, 2017, 109: 11-17.
[9]	Azeem M, Arockiam Jeyasundar PGS, Ali A, et al. Cow bone-derived biochar enhances microbial biomass and alters bacterial community composition and diversity in a smelter contaminated soil[J]. Environ Res, 2023, 216(Pt 1): 114278.
[10]	Wang H, Huang QN, Zhang Y, et al. Biochar decreases soil cadmium availability and regulates expression levels of Cd uptake/transport-related genes to reduce Cd translocation in rice[J]. Rice Sci, 2024
[11]	Peng Q, Wang P, Yang C, et al. Remediation effect of walnut shell biochar on Cu and Pb co-contaminated soils in different utilization types[J]. J Environ Manag, 2024, 362: 121322.
[12]	Li SS, Wang PP, Liu XG, et al. Polyoxymethylene passive samplers to assess the effectiveness of biochar by reducing the content of freely dissolved fipronil and ethiprole[J]. Sci Total Environ, 2018, 630: 960-966.
[13]	陈浩宇, 段友丽. 不同生物炭对水中氨氮的吸附特性及影响因素对比[J]. 净水技术, 2022, 41(6): 71-78, 95.
	Chen HY, Duan YL. Contrast of adsorption property and influencing factors of different biochar for ammonia nitrogen removal in water[J]. Water Purif Technol, 2022, 41(6): 71-78, 95.
[14]	Tu C, Wei J, Guan F, et al. Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil[J]. Environ Int, 2020, 137: 105576.
[15]	张倩茹, 冀琳宇, 高程程, 等. 改性生物炭的制备及其在环境修复中的应用[J]. 农业环境科学学报, 2021, 40(5): 913-925.
	Zhang QR, Ji LY, Gao CC, et al. Preparation of modified biochar and its application in environmental remediation[J]. J Agro Environ Sci, 2021, 40(5): 913-925.
[16]	Muneer B, Iqbal MJ, Shakoori FR, et al. Isolation, identification and cadmium processing of Pseudomonas aeruginosa(EP-Cd1)isolated from soil contaminated with electroplating industrial wastewater[J]. Pakistan Journal of Zoology, 2016, 48(5):1495-1501.
[17]	Taha M, Shahsavari E, Aburto-Medina A, et al. Bioremediation of biosolids with Phanerochaete chrysosporium culture filtrates enhances the degradation of polycyclic aromatic hydrocarbons(PAHs)[J]. Appl Soil Ecol, 2018, 124: 163-170.
[18]	Rivelli V, Franzetti A, Gandolfi I, et al. Persistence and degrading activity of free and immobilised allochthonous bacteria during bioremediation of hydrocarbon-contaminated soils[J]. Biodegradation, 2013, 24(1): 1-11. doi: 10.1007/s10532-012-9553-x pmid: 22555628
[19]	Pérez Vargas J, Viguera Carmona SE, Zamudio Moreno E, et al. Bioremediation of soils from oil spill impacted sites using bioaugmentation with biosurfactants producing, native, free-living nitrogen fixing bacteria[J]. Rev Int Contam Ambie, 2017, 33(esp01): 105-114.
[20]	Gao MZ, Ling N, Tian HY, et al. Toxicity, physiological response, and biosorption mechanism of Dunaliella salina to copper, lead, and cadmium[J]. Front Microbiol, 2024, 15: 1374275.
[21]	Wang LL, Liu YM, Shu XL, et al. Complexation and conformation of lead ion with poly-γ-glutamic acid in soluble state[J]. PLoS One, 2019, 14(9): e0218742.
[22]	何稼, 楚剑, 刘汉龙, 等. 微生物岩土技术的研究进展[J]. 岩土工程学报, 2016, 38(4): 643-653.
	He J, Chu J, Liu HL, et al. Research advances in biogeotechnologies[J]. Chin J Geotech Eng, 2016, 38(4): 643-653.
[23]	Cid-Barrio L, Bouzas-Ramos D, Salinas-Castillo A, et al. Quantitative assessment of cellular uptake and differential toxic effects of HgSe nanoparticles in human cells[J]. J Anal at Spectrom, 2020, 35(9): 1979-1988.
[24]	Huang JH. Impact of microorganisms on arsenic biogeochemistry: a review[J]. Water Air Soil Pollut, 2014, 225(2): 1848.
[25]	姜恒丽, 崔元璐, 齐学洁, 等. 海藻酸钠-壳聚糖微胶囊载体在组织工程研究中的应用[J]. 中国组织工程研究, 2014, 18(3): 412-419.
	Jiang HL, Cui YL, Qi XJ, et al. Alginate-chitosan microcapsule in tissue engineering research[J]. Chin J Tissue Eng Res, 2014, 18(3): 412-419.
[26]	Zhu XM, Chen BL, Zhu LZ, et al. Effects and mechanisms of biochar-microbe interactions in soil improvement and pollution remediation: a review[J]. Environ Pollut, 2017, 227: 98-115. doi: S0269-7491(16)32228-X pmid: 28458251
[27]	Bai XF, Li ZF, Zhang YZ, et al. Recovery of ammonium in urine by biochar derived from faecal sludge and its application as soil conditioner[J]. Waste Biomass Valorization, 2018, 9(9): 1619-1628.
[28]	金明兰, 赵海川, 李华南, 等. 秸秆生物炭与生物菌剂协同处理土壤中重金属和抗生素的研究[J/OL]. 北方园艺, 2024. http://kns.cnki.net/kcms/detail/23.1247.s.20240409.0858.003.html.
	Jin ML, Zhao HC, Li HN, et al. Biochar and microbial agents soil synergistic treatment of heavy metals and antibiotics in pollutant soil by straw[J/OL]. Northern Horticulture, 2024. http://kns.cnki.net/kcms/detail/23.1247.s.20240409.0858.003.html.
[29]	Samonin VV, Elikova EE. A study of the adsorption of bacterial cells on porous materials[J]. Mikrobiologiia, 2004, 73(6): 810-816. pmid: 15688940
[30]	Abit SM, Bolster CH, Cai P, et al. Influence of feedstock and pyrolysis temperature of biochar amendments on transport of Escherichia coli in saturated and unsaturated soil[J]. Environ Sci Technol, 2012, 46(15): 8097-8105.
[31]	Sathishkumar K, Li Y, Sanganyado E. Electrochemical behavior of biochar and its effects on microbial nitrate reduction: role of extracellular polymeric substances in extracellular electron transfer[J]. Chem Eng J, 2020, 395: 125077.
[32]	Flowers L, Ohnishi ST, Penning TM. DNA strand scission by polycyclic aromatic hydrocarbon o-quinones: role of reactive oxygen species, Cu(II)/Cu(I)redox cycling, and o-semiquinone anion radicals[J]. Biochemistry, 1997, 36(28): 8640-8648. pmid: 9214311
[33]	Kizito S, Luo HZ, Lu JX, et al. Role of nutrient-enriched biochar as a soil amendment during maize growth: exploring practical alternatives to recycle agricultural residuals and to reduce chemical fertilizer demand[J]. Sustainability, 2019, 11(11): 3211.
[34]	Łapczyńska-Kordon B, Ślipek Z, Słomka-Polonis K, et al. Physicochemical properties of biochar produced from goldenrod plants[J]. Materials(Basel), 2022, 15(7): 2615.
[35]	Kloss S, Zehetner F, Dellantonio A, et al. Characterization of slow pyrolysis biochars: effects of feedstocks and pyrolysis temperature on biochar properties[J]. J Environ Qual, 2012, 41(4): 990-1000. doi: 10.2134/jeq2011.0070 pmid: 22751041
[36]	Zheng ZJ, Ali A, Su JF, et al. Self-immobilized biochar fungal pellet combined with bacterial strain H29 enhanced the removal performance of cadmium and nitrate[J]. Bioresour Technol, 2021, 341: 125803.
[37]	Wang L, Chen HR, Wu JZ, et al. Effects of magnetic biochar-microbe composite on Cd remediation and microbial responses in paddy soil[J]. J Hazard Mater, 2021, 414: 125494.
[38]	Zhang Y, Yin QX, Guo LL, et al. Chicken manure-derived biochar enhanced the potential of Comamonas testosteroni ZG2 to remediate Cd contaminated soil[J]. Environ Geochem Health, 2024, 46(6): 198.
[39]	Qi WY, Chen H, Wang Z, et al. Biochar-immobilized Bacillus megaterium enhances Cd immobilization in soil and promotes Brassica chinensis growth[J]. J Hazard Mater, 2023, 458: 131921.
[40]	Qi X, Xiao SQ, Chen XM, et al. Biochar-based microbial agent reduces U and Cd accumulation in vegetables and improves rhizosphere microecology[J]. J Hazard Mater, 2022, 436: 129147.
[41]	Chang JJ, Duan YJ, Dong J, et al. Bioremediation of Hg-contaminated soil by combining a novel Hg-volatilizing Lecythophora sp. fungus, DC-F1, with biochar: performance and the response of soil fungal community[J]. Sci Total Environ, 2019, 671: 676-684.
[42]	Huang YT, Liu T, Liu J, et al. Exceptional anti-toxic growth of water spinach in arsenic and cadmium co-contaminated soil remediated using biochar loaded with Bacillus aryabhattai[J]. J Hazard Mater, 2024, 469: 133966.
[43]	Xiong BJ, Zhang YC, Hou YW, et al. Enhanced biodegradation of PAHs in historically contaminated soil by M. gilvum inoculated biochar[J]. Chemosphere, 2017, 182: 316-324.
[44]	Zhou X, Sun YH, Wang TT, et al. Remediation potential of an immobilized microbial consortium with corn straw as a carrier in polycyclic aromatic hydrocarbons contaminated soil[J]. J Hazard Mater, 2024, 469: 134091.
[45]	Lu JF, Liu YX, Zhang RL, et al. Biochar inoculated with Pseudomonas putida alleviates its inhibitory effect on biodegradation pathways in phenanthrene-contaminated soil[J]. J Hazard Mater, 2024, 461: 132550.
[46]	Cui CZ, Shen JM, Zhu Y, et al. Bioremediation of phenanthrene in saline-alkali soil by biochar- immobilized moderately halophilic bacteria combined with Suaeda salsa L[J]. Sci Total Environ, 2023, 880: 163279.
[47]	Song LC, Niu XG, Zhou B, et al. Application of biochar-immobilized Bacillus sp. KSB7 to enhance the phytoremediation of PAHs and heavy metals in a coking plant[J]. Chemosphere, 2022, 307(Pt 4): 136084.
[48]	Wang CH, Gu LF, Ge SM, et al. Remediation potential of immobilized bacterial consortium with biochar as carrier in Pyrene-Cr(VI)co-contaminated soil[J]. Environ Technol, 2019, 40(18): 2345-2353.
[49]	Qi X, Zhu MH, Yuan YB, et al. Bioremediation of PBDEs and heavy metals co-contaminated soil in e-waste dismantling sites by Pseudomonas plecoglossicida assisted with biochar[J]. J Hazard Mater, 2023, 460: 132408.
[50]	Takagi K. Study on the biodegradation of persistent organic pollutants(POPs)[J]. J Pestic Sci, 2020, 45(2): 119-123.
[51]	Bargaz A, Elhaissoufi W, Khourchi S, et al. Benefits of phosphate solubilizing bacteria on belowground crop performance for improved crop acquisition of phosphorus[J]. Microbiol Res, 2021, 252: 126842.
[52]	Wang CX, Ren J, Qiao X, et al. Ammonium removal efficiency of biochar-based heterotrophic nitrifying bacteria immobilization body in water solution[J]. Environ Eng Res, 2021, 26(1): 190451.
[53]	Shao Y, Zhong H, Mao X, et al. Biochar-immobilized Sphingomonas sp. and Acinetobacter sp. isolates to enhance nutrient removal: potential application in crab aquaculture[J]. Aquacult Environ Interact, 2020, 12: 251-262.
[54]	Sun PF, Huang X, Xing YX, et al. Immobilization of Ochrobactrum sp. on biochar/clay composite particle: optimization of preparation and performance for nitrogen removal[J]. Front Microbiol, 2022, 13: 838836.
[55]	赏国锋, 张涵, 沈逸菲, 等. 生物炭固定化硝化菌去除水样中氨氮的研究[J]. 上海交通大学学报: 农业科学版, 2014, 32(5): 44-47.
	Shang GF, Zhang H, Shen YF, et al. Removal of ammonia nitrogen in aqueous samples by biochar immobilized nitrifying bacteria[J]. J Shanghai Jiao Tong Univ Agric Sci, 2014, 32(5): 44-47.
[56]	吴梦莉, 李洁, 智燕彩, 等. 微生物固定化生物炭对水体铵态氮去除效果的研究[J]. 农业环境科学学报, 2021, 40(5): 1071-1078.
	Wu ML, Li J, Zhi YC, et al. Synthesis of microbial immobilized biochar for the removal of ammonia nitrogen from aqueous solutions[J]. J Agro Environ Sci, 2021, 40(5): 1071-1078.
[57]	An Q, Ran BB, Deng SM, et al. Peanut shell biochar immobilized Pseudomonas hibiscicola strain L1 to remove electroplating mixed-wastewater[J]. J Environ Chem Eng, 2023, 11(2): 109411.
[58]	Zhang SN, Wang JH, Wang SH, et al. Effective removal of chlortetracycline and treatment of simulated sewage by Bacillus cereus LZ01 immobilized on erding medicine residues biochar[J]. Biomass Convers Biorefin, 2024, 14(2): 2281-2291.
[59]	Sonsuphab K, Toomsan W, Supanchaiyamat N, et al. Enhanced triclocarban remediation from groundwater using Pseudomonas fluorescens strain MC46 immobilized on agro-industrial waste-derived biochar: optimization and kinetic analysis[J]. J Environ Chem Eng, 2022, 10(3): 107610.
[60]	Zhang SN, Wang JH. Removal of chlortetracycline from water by Bacillus cereus immobilized on Chinese medicine residues biochar[J]. Environ Technol Innov, 2021, 24: 101930.
[61]	Zhang SN, Wang JH. Removal of chlortetracycline from water by immobilized Bacillus subtilis on honeysuckle residue-derived biochar[J]. Water Air Soil Pollut, 2021, 232(6): 236.
[62]	Xia MM, Niu QY, Qu XY, et al. Simultaneous adsorption and biodegradation of oxytetracycline in wastewater by Mycolicibacterium sp. immobilized on magnetic biochar[J]. Environ Pollut, 2023, 339: 122728.
[63]	Zhang SD, Hou JJ, Zhang XT, et al. Biochar-assisted degradation of oxytetracycline by Achromobacter denitrificans and underlying mechanisms[J]. Bioresour Technol, 2023, 387: 129673.
[64]	Li YY, Zhu YE, Yan XR, et al. Strategy and mechanisms of sulfamethoxazole removal from aqueous systems by single and combined Shewanella oneidensis MR-1 and nanoscale zero-valent iron-enriched biochar[J]. Sci Total Environ, 2023, 883: 163676.
[65]	Chen X, Lin H, Dong YB, et al. Mechanisms underlying enhanced bioremediation of sulfamethoxazole and zinc(II)by Bacillus sp. SDB4 immobilized on biochar[J]. J Clean Prod, 2022, 370: 133483.
[66]	Nguyen AH, Youn S, Yang YY, et al. Alkaline-modified biochar and nitrifying microbiome synergistically mitigate the toxicity of oxytetracycline and its toxic by-products[J]. Chem Eng J, 2024, 481: 148527.
[67]	Liang EL, Xu L, Su JF, et al. Nano iron tetroxide-modified rice husk biochar promoted Feammox performance of Klebsiella sp. FC61 and synergistically removed Ni²⁺ and ciprofloxacin[J]. Bioresour Technol, 2023, 382: 129183.
[68]	Hargreaves JA. Nitrogen biogeochemistry of aquaculture ponds[J]. Aquaculture, 1998, 166(3/4): 181-212.
[69]	洪枫, 吴春香. 亚硝酸盐在小棚虾养殖中的危害与防治[J]. 科学养鱼, 2024(3): 60-61.
	Hong F, Wu CX. Harm and control of nitrite in shrimp culture in small shed[J]. Sci Fish Farming, 2024(3): 60-61.
[70]	Cheng ZW, Chen JM, Chen DZ, et al. Biodegradation of methyl Tert-butyl ether in a bioreactor using immobilized Methylibium petroleiphilumPM1 cells[J]. Water Air Soil Pollut, 2011, 214(1): 59-72.
[71]	郑惠东. 水环境中抗生素来源及对健康的影响[J]. 环境卫生学杂志, 2018, 8(1): 73-77.
	Zheng HD. The source of antibiotics in aquatic environment and its impact on human health[J]. J Environ Hyg, 2018, 8(1): 73-77.
[72]	Wani AK, Akhtar N, Sher F, et al. Microbial adaptation to different environmental conditions: molecular perspective of evolved genetic and cellular systems[J]. Arch Microbiol, 2022, 204(2): 144. doi: 10.1007/s00203-022-02757-5 pmid: 35044532
[73]	Oni BA, Oziegbe O, Olawole OO. Significance of biochar application to the environment and economy[J]. Ann Agric Sci, 2019, 64(2): 222-236.
[74]	Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research[J]. Nature, 2014, 507(7491): 181-189.
[75]	Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9[J]. Science, 2014, 346(6213): 1258096.
[76]	Quince C, Walker AW, Simpson JT, et al. Shotgun metagenomics, from sampling to analysis[J]. Nat Biotechnol, 2017, 35(9): 833-844. doi: 10.1038/nbt.3935 pmid: 28898207

污染物 Pollutant	功能微生物 Microorganism	生物炭类型 Biochar type	负载方式 Loaded method	去除率 Removal ratio/%	参考文献 Reference
镉 Cd	芽孢杆菌Bacillus sp. K1	磁性稻草秸秆Magnetic rice straw	包埋法Embedding	88.1	[37]
镉 Cd	丛毛单胞菌Testosteroni ZG2	鸡的粪便衍生物 Chicken manure-derived	吸附法Adsorption	81.4	[38]
镉 Cd	芽孢杆菌Megaterium	玉米秸秆Maize straw	吸附法Adsorption	25.53	[39]
镉 Cd	MA菌群（Bacillus subtilis, B. cereus, and Citrobacter sp.）	玉米秸秆Maize straw	吸附法Adsorption	54.2	[40]
汞 Hg	Lecythophora sp. DC-F1	松木Pine sawdust	混合法Hybridization	13.3-26.1	[41]
砷 As	Bacillus aryabhattai	贝壳Shell	吸附法Adsorption	63.51	[42]
菲 PHE	Mycobacterium gilvum	水稻秸秆、污泥、猪粪 Rice straw, sewage sludge, and pig manure	吸附法Adsorption	62.6	[43]
16 US-EPA PAHs	J-3菌群（主要含Shinella, Azospirillum,and Rhodospirillales_norank）	玉米秸秆Corn straw	吸附法Adsorption	88.25	[44]
菲 PHE	Pseudomonas putida	竹子Bamboo	吸附法Adsorption	96.82	[45]
菲 PHE	Suaeda salsa L.	棉秆Cotton stalk	吸附法Adsorption	91.67	[46]
多环芳烃 PAHs	Bacillus sp. KSB7	花生壳Peanut shell	吸附法Adsorption	36.7	[47]
芘Pyrene	Bacillus sp. W1 Bacillus sp. W2	菠萝皮Pineapple peels	吸附法Adsorption	82.32	[48]
2,2',4,4'四溴化二苯醚 BDE-47	假单胞菌Plecoglossicida	水葫芦Eichhornia crassipes	吸附法Adsorption	42.8	[49]
六氯环己烷 α-HCH	鞘氨醇单胞菌Sphingomonas（TSK-1）诺卡菌Nocardioides（PD653）	椰子壳Coconut shell	吸附法Adsorption	67.1	[50]

污染物 Pollutant	功能微生物 Microorganism	生物炭类型 Biochar type	负载方式 Loaded method	去除率 Removal ratio/%	参考文献 Reference
镉 Cd	芽孢杆菌Bacillus sp. K1	磁性稻草秸秆Magnetic rice straw	包埋法Embedding	88.1	[37]
镉 Cd	丛毛单胞菌Testosteroni ZG2	鸡的粪便衍生物 Chicken manure-derived	吸附法Adsorption	81.4	[38]
镉 Cd	芽孢杆菌Megaterium	玉米秸秆Maize straw	吸附法Adsorption	25.53	[39]
镉 Cd	MA菌群（Bacillus subtilis, B. cereus, and Citrobacter sp.）	玉米秸秆Maize straw	吸附法Adsorption	54.2	[40]
汞 Hg	Lecythophora sp. DC-F1	松木Pine sawdust	混合法Hybridization	13.3-26.1	[41]
砷 As	Bacillus aryabhattai	贝壳Shell	吸附法Adsorption	63.51	[42]
菲 PHE	Mycobacterium gilvum	水稻秸秆、污泥、猪粪 Rice straw, sewage sludge, and pig manure	吸附法Adsorption	62.6	[43]
16 US-EPA PAHs	J-3菌群（主要含Shinella, Azospirillum,and Rhodospirillales_norank）	玉米秸秆Corn straw	吸附法Adsorption	88.25	[44]
菲 PHE	Pseudomonas putida	竹子Bamboo	吸附法Adsorption	96.82	[45]
菲 PHE	Suaeda salsa L.	棉秆Cotton stalk	吸附法Adsorption	91.67	[46]
多环芳烃 PAHs	Bacillus sp. KSB7	花生壳Peanut shell	吸附法Adsorption	36.7	[47]
芘Pyrene	Bacillus sp. W1 Bacillus sp. W2	菠萝皮Pineapple peels	吸附法Adsorption	82.32	[48]
2,2',4,4'四溴化二苯醚 BDE-47	假单胞菌Plecoglossicida	水葫芦Eichhornia crassipes	吸附法Adsorption	42.8	[49]
六氯环己烷 α-HCH	鞘氨醇单胞菌Sphingomonas（TSK-1）诺卡菌Nocardioides（PD653）	椰子壳Coconut shell	吸附法Adsorption	67.1	[50]

污染物 Pollutant	功能微生物 Microorganisms	生物炭类型 Biochar type	负载方式 Loaded method	去除率 Removal ratio/%	参考文献 Reference
氨氮 NH₄⁺-N	异养硝化细菌 Heterotrophic nitrifying bacteria	稻壳衍生 Rice husk-derived	吸附法 Adsorption	90.93	[52]
氨氮 NH₄⁺-N	鞘氨单胞菌 Sphingomonas sp.	玉米秸秆 Maize straw	吸附法 Adsorption	63	[53]
氨氮 NH₄⁺-N	苍白杆菌 Ochrobactrum sp.	芦苇秸秆 Reed straw	包埋法 Embedding	79.39	[54]
氨氮 NH₄⁺-N	硝化细菌 Nitrifying bacteria	稻壳衍生 Rice hull derived	包埋法 Embedding	85	[55]
氨氮 NH₄⁺-N	脱氮副球菌、假单胞菌和拉乌尔菌 Paracoccus denitrificans, Pseudomonas and Raoultella	花生壳 Peanut shell	吸附法包埋法 Adsorption Embedding	97.9-99.1	[56]
氨氮 NH₄⁺-N	木槿假单胞菌L1菌 Pseudomonas hibiscicola strain L1	花生壳 Peanut shell	吸附法 Adsorption	99.99	[57]
氯四环素 Chlortetracycline	蜡样芽孢杆菌 Bacillus cereus	药渣 Erding medicine residues	吸附法 Adsorption	83.83	[58]
三氯二苯脲 Initial triclocarban	荧光假单胞菌 Pseudomonas fluorescens strain MC46	桉树枝 Eucalyptus branches	吸附法 Adsorption	96	[59]
氯四环素 Chlortetracycline	蜡样芽孢杆菌 Bacillus cereus	中药渣 Chinese medicine residues	吸附法 Adsorption	85.42 ± 0.82	[60]
氯四环素 Chlortetracycline	枯草芽孢杆菌 Bacillus subtilis	金银花残渣 Honeysuckle residue-derived	吸附法 Adsorption	78.35	[61]
土霉素 Oxytetracycline	分枝杆菌 Mycolicibacterium sp.	磁性 Magnetic	吸附法 Adsorption	95.7	[62]
土霉素 Oxytetracycline	反硝化无色杆菌 Achromobacter denitrificans	秸秆 Rice straw	吸附法 Adsorption	95.01-100	[63]
磺胺甲噁唑 Sulfamethoxazole	希瓦氏菌MR-1 Shewanella oneidensis MR-1	纳米级零价富铁 Nanoscale zero-valent iron-enriched	吸附法 Adsorption	100	[64]
磺胺甲噁唑 Sulfamethoxazole	副霉芽孢杆菌SDB4 Bacillus sp. SDB4	猪粪 Pig manure	吸附法 Adsorption	100	[65]
土霉素 Oxytetracycline	硝化微生物组 Nitrifying microbiome	碱性改性 Alkaline-modified	包埋法 Embedding method	>95	[66]
环丙沙星 Ciprofloxacin	克雷伯氏菌FC61 Klebsiella sp. FC61	纳米四氧化铁改性稻壳Nano iron tetroxide-modified rice husk	吸附法 Adsorption method	81.82	[67]

污染物 Pollutant	功能微生物 Microorganisms	生物炭类型 Biochar type	负载方式 Loaded method	去除率 Removal ratio/%	参考文献 Reference
氨氮 NH₄⁺-N	异养硝化细菌 Heterotrophic nitrifying bacteria	稻壳衍生 Rice husk-derived	吸附法 Adsorption	90.93	[52]
氨氮 NH₄⁺-N	鞘氨单胞菌 Sphingomonas sp.	玉米秸秆 Maize straw	吸附法 Adsorption	63	[53]
氨氮 NH₄⁺-N	苍白杆菌 Ochrobactrum sp.	芦苇秸秆 Reed straw	包埋法 Embedding	79.39	[54]
氨氮 NH₄⁺-N	硝化细菌 Nitrifying bacteria	稻壳衍生 Rice hull derived	包埋法 Embedding	85	[55]
氨氮 NH₄⁺-N	脱氮副球菌、假单胞菌和拉乌尔菌 Paracoccus denitrificans, Pseudomonas and Raoultella	花生壳 Peanut shell	吸附法包埋法 Adsorption Embedding	97.9-99.1	[56]
氨氮 NH₄⁺-N	木槿假单胞菌L1菌 Pseudomonas hibiscicola strain L1	花生壳 Peanut shell	吸附法 Adsorption	99.99	[57]
氯四环素 Chlortetracycline	蜡样芽孢杆菌 Bacillus cereus	药渣 Erding medicine residues	吸附法 Adsorption	83.83	[58]
三氯二苯脲 Initial triclocarban	荧光假单胞菌 Pseudomonas fluorescens strain MC46	桉树枝 Eucalyptus branches	吸附法 Adsorption	96	[59]
氯四环素 Chlortetracycline	蜡样芽孢杆菌 Bacillus cereus	中药渣 Chinese medicine residues	吸附法 Adsorption	85.42 ± 0.82	[60]
氯四环素 Chlortetracycline	枯草芽孢杆菌 Bacillus subtilis	金银花残渣 Honeysuckle residue-derived	吸附法 Adsorption	78.35	[61]
土霉素 Oxytetracycline	分枝杆菌 Mycolicibacterium sp.	磁性 Magnetic	吸附法 Adsorption	95.7	[62]
土霉素 Oxytetracycline	反硝化无色杆菌 Achromobacter denitrificans	秸秆 Rice straw	吸附法 Adsorption	95.01-100	[63]
磺胺甲噁唑 Sulfamethoxazole	希瓦氏菌MR-1 Shewanella oneidensis MR-1	纳米级零价富铁 Nanoscale zero-valent iron-enriched	吸附法 Adsorption	100	[64]
磺胺甲噁唑 Sulfamethoxazole	副霉芽孢杆菌SDB4 Bacillus sp. SDB4	猪粪 Pig manure	吸附法 Adsorption	100	[65]
土霉素 Oxytetracycline	硝化微生物组 Nitrifying microbiome	碱性改性 Alkaline-modified	包埋法 Embedding method	>95	[66]
环丙沙星 Ciprofloxacin	克雷伯氏菌FC61 Klebsiella sp. FC61	纳米四氧化铁改性稻壳Nano iron tetroxide-modified rice husk	吸附法 Adsorption method	81.82	[67]