Effect of Exogenous Salicylic Acid on Wheat Infested with Blumeria graminis f. sp. tritici and Its Transcriptome Analysis

doi:10.13560/j.cnki.biotech.bull.1985.2024-1201

Abstract

Abstract:

Objective Powdery mildew, one of the most devastating diseases affecting wheat, poses a significant threat to wheat yield and quality. This study aims to investigate the regulatory effects of exogenous salicylic acid (SA) on Blumeria graminis f. sp. tritici (abbreviated as Bgt below) infection and to reveal the molecular mechanisms underlying Bgt infection and SA-mediated resistance to disease through transcriptomic analysis. Method In this experiment, we analyzed the transcriptome of wheat powdery mildew fungus under Bgt-infested conditions for 1-4 d using powdery mildew-susceptible common wheat Zhongzuo 9504 as the material. Concurrently, salicylic acid was applied externally to observe the effect of phytohormones on powdery mildew-infected wheat. Result Transcriptomic analysis revealed that, compared to 1 d, 399 genes were upregulated and 1 110 genes were downregulated at 4 d. The upregulated genes were enriched in pathways such as secondary metabolite biosynthesis, metabolic pathways, and proteasome, while the downregulated genes were primarily involved in the degradation of valine, leucine, and isoleucine. Protein-protein interaction network analysis identified three core hub genes of Bgt: BGTH12_LOCUS642, BGTH12_LOCUS3045, and BGTH12_LOCUS5497, indicating their crucial roles during wheat infection. Furthermore, exogenous SA significantly upregulated the expressions of six hub genes in wheat (ERF109, PP2C30, TIFY6B, HSP70, At4g15970, and HERK1), thereby reducing the damage caused by Bgt. Conclusion During the infection process, Bgt demonsttrates extensive differential gene expression, with BGTH12_LOCUS642, BGTH12_LOCUS3045, and BGTH12_LOCUS5497 identified as core hub genes. The external application of salicylic acid may induce the expressions of resistance genes and inhibit the growth and development of conidia under the conditions of powdery mildew infection, thereby slowing down the infection of powdery mildew to a certain extent and increasing the resistance of wheat to powdery mildew.

Key words: wheat powdery mildew, Blumeria graminis f. sp. tritici, exogenous salicylic acid, transcriptome analysis

LI Cheng-hua, DOU Fei-fei, REN Yu-zhao, LIU Cai-xia, LIU Feng-lou, WANG Zhang-jun, LI Qing-feng. Effect of Exogenous Salicylic Acid on Wheat Infested with Blumeria graminis f. sp. tritici and Its Transcriptome Analysis[J]. Biotechnology Bulletin, 2025, 41(7): 272-280.

Figures/Tables 8

References 36

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基因名称 Gene name	基因ID Gene ID	正向引物 Forward primer （5′-3′）	反向引物 Reverse primer （5′-3′）
ERF109	TraesCS1A02G370700.1	AGGGGCACGAGTACATGATC	AGCTTCATCAGGTCCTGCAG
T7H20-70	TraesCS1D02G280200.2	TGGACGCCAAGAAGAGGTC	CTTGCCACCAATCACCTCTG
CIGR2	TraesCS2A02G189600.1	ATCCACCCCTTCAGCAACAT	GAGGGGCTTGCTCTTCCAG
HSP70	TraesCS4B02G205700.1	TCGGCACCACATACTCCTG	CAGTCCACTTCTTCGATCTTGG
PP2C30	TraesCS4B02G210100.1	ATGTCAGAGATCCGCCGC	GTTGTCCGAGCTCTGCCG
TIFY6B	TraesCS5A02G204900.1	GGAGTCAGCTTACTTTGGGG	CTAAAGCTGATGTTCCTGGGC
DUF4228	TraesCS5D02G414000.1	ACGGCGAGGGTGGTTCTC	GAGATGGCGGTTAGGTCGG
At4g15970	TraesCS5D02G536500.1	CAGCAGCAACATTAGCCCC	CACGTCCAACACATTCCTCA
tmem53	TraesCS7B02G502200.1	AAACTCTCCCACCACCACAG	CGCAGTCCTCCAAGAAGTTG
HERK1	TraesCS7D02G338800.3	CTCTACTCCCCTTTCCGCTG	TCACCTCCCCTCAGCTTTG
FLA17	TraesCS2B02G263900.1	TCCTCCTCTGCCTGGTACT	CCTTCCAACAACTTCCCGC
Actin	-	TACTCCCTCACAACAACCG	AACAAGAGTCACCTCCAAGA

基因名称 Gene name	基因ID Gene ID	正向引物 Forward primer （5′-3′）	反向引物 Reverse primer （5′-3′）
ERF109	TraesCS1A02G370700.1	AGGGGCACGAGTACATGATC	AGCTTCATCAGGTCCTGCAG
T7H20-70	TraesCS1D02G280200.2	TGGACGCCAAGAAGAGGTC	CTTGCCACCAATCACCTCTG
CIGR2	TraesCS2A02G189600.1	ATCCACCCCTTCAGCAACAT	GAGGGGCTTGCTCTTCCAG
HSP70	TraesCS4B02G205700.1	TCGGCACCACATACTCCTG	CAGTCCACTTCTTCGATCTTGG
PP2C30	TraesCS4B02G210100.1	ATGTCAGAGATCCGCCGC	GTTGTCCGAGCTCTGCCG
TIFY6B	TraesCS5A02G204900.1	GGAGTCAGCTTACTTTGGGG	CTAAAGCTGATGTTCCTGGGC
DUF4228	TraesCS5D02G414000.1	ACGGCGAGGGTGGTTCTC	GAGATGGCGGTTAGGTCGG
At4g15970	TraesCS5D02G536500.1	CAGCAGCAACATTAGCCCC	CACGTCCAACACATTCCTCA
tmem53	TraesCS7B02G502200.1	AAACTCTCCCACCACCACAG	CGCAGTCCTCCAAGAAGTTG
HERK1	TraesCS7D02G338800.3	CTCTACTCCCCTTTCCGCTG	TCACCTCCCCTCAGCTTTG
FLA17	TraesCS2B02G263900.1	TCCTCCTCTGCCTGGTACT	CCTTCCAACAACTTCCCGC
Actin	-	TACTCCCTCACAACAACCG	AACAAGAGTCACCTCCAAGA