Biotechnology Bulletin ›› 2012, Vol. 0 ›› Issue (12): 101-105.

• Study Report • Previous Articles     Next Articles

Bt New Gene sip Cloning, Expression and Bioinformatics Analysis

Liu Yanjie, Li Haitao, Liu Rongmei, Gao Jiguo   

  1. Life Science College of Northeast Agricultural University, Harbin 150030 )
  • Received:2012-05-28 Revised:2013-01-25 Online:2012-12-26 Published:2013-02-06

Abstract: Bacillus thuringiensis is the most widely applied type of microbial pesticides because of its high specificity and environmentalsafety. There is a wide range of researchs about ICPs and Vip, but little was known about Sip. To further explore novel Bacillus thuringiensisstrains and the new gene resources in our country, we isolated 400 wild strains from different entironment for sip gene cloning and identification,of which amplification from QZL26 isolate got a sequence of 1 038 bp which codes 345 amino acids and the similarity of the amino acid sequencewith the Sip1A is 91.83%. Sequencing result was submitted to GenBank, and the registration number is JQ965994.