Abstract: This study aimed to clone the full length cDNA of goat PHGPx gene. Total RNA was extracted from goat testis and the fulllength cDNA was obtained by the reverse transcription PCR and RACE method. The results demonstrated that the full length of goat PHGPxcDNA was 844 bp, which encode 199 amino acids. The multiple sequence alignments revealed that the identity of goat PHGPx amino acidssequence were above 90% with other species. Bioinformatics analysis indicated that goat PHGPx belonged to GSH-Px family in the secondarystructure, and predicted the signal peptide at the position of 23-24 or 28-29. The molecular phylogenetic trees among species according theUPGMA method indicated that goat and cow assembled, and then assembled with pig, rat, human and chicken, respectively and assembled withbee last. This result of phylogenetic clustering was identical to the zoological classification. In summary, Capra hircus PHGPx gene has beencloned, and it’s concluded that PHGPx has typical characteristics with the homologous genes in GSH-Px family. These results would be thetheoretical foundation for the further study on the genetic control of PHGPx gene expression.