Study of Arabidopsis BAND7 Gene Sequence Analysis and MutantPhysiological Function

Biotechnology Bulletin ›› 2012, Vol. 0 ›› Issue (12): 120-124.

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Study of Arabidopsis BAND7 Gene Sequence Analysis and MutantPhysiological Function

Sun Hongju, Xue Qiong, Zhang Yantao, Wang Yan, Kang Yan, Qi Zhi

Inner Mongolia University，College of Life Sciences，Hohhot 010021

Received:2012-09-29 Revised:2013-01-25 Online:2012-12-26 Published:2013-02-06

Abstract

Abstract: BAND7 is one kind of proteins with SPFH structural motif and wildly distributed in various animal cells, which coupledwith other membrane proteins and regulated ion transportation and signaling transduction. By sequencing comparison, four putative BAND7genes were identified in Arabidopsis, At1g69840, At3g01290, At5g6274 and At5g51570. Semi-quantitative reverse transcript PCR analysisindicated that they expressed in both the leaf and root with comparable higher expression in the leaf. Through PCR-based detection method,T-DNA knockout homozygous mutants SALK_088328 and SALK_104547 were identified in the At1g69840 and At5g51570 genes, respectively.Comparing growth phenotype of the wild type and these mutants revealed a role of At5g51570 in the calcium homeostasis.

Sun Hongju, Xue Qiong, Zhang Yantao, Wang Yan, Kang Yan, Qi Zhi . Study of Arabidopsis BAND7 Gene Sequence Analysis and MutantPhysiological Function[J]. Biotechnology Bulletin, 2012, 0(12): 120-124.

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Study of Arabidopsis BAND7 Gene Sequence Analysis and MutantPhysiological Function

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