Research Progress on Spermatozoa-mediated Gene Transfer to Product Transgenic Animals

Abstract

Abstract: Spermatozoa-mediated gene transfer（SMGT）is a low-cost and fast method used to produce transgenic animals. Theexogenous DNA can be either integrated into the sperm chromosomal DNA or simply transferred to the egg by the spermatozoa and laterintegrated into the zygote’s genome. Although there are many transgenic animals produced by SMGT，its efficiency is still low，mainly dueto the ability of sperm to bind，internalize，and transport exogenous DNA into an oocyte during fertilization. The purpose of this review is tosummarize what has been achieved with this method，such as the binding of DNA molecules to spermatozoa，and attempts made to producetransgenic animals in this field.

Du Wei, Cui Haixin, Wang Yan, Sun Changjiao, Zhao Xiang . Research Progress on Spermatozoa-mediated Gene Transfer to Product Transgenic Animals[J]. Biotechnology Bulletin, 2012, 0(12): 13-18.

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