Biotechnology Bulletin ›› 2012, Vol. 0 ›› Issue (12): 156-162.

• Study Report • Previous Articles     Next Articles

Isolation,Identification and Characterization of a Crude-oildegradatingThermophiles in Petroleum Reservoirs

He Hua1,2, Qiao Fadong2, An Mingli1, He Weihong1, Du Xun1, Jia Bin1, Wang Yanan1   

  1. 1College of Bioengineering,Henan University of Technology,Zhengzhou 450008 ;2Key Laboratory of Microbial Engineering of HenanProvince,Zhengzhou 450001
  • Received:2012-05-29 Revised:2013-01-25 Online:2012-12-26 Published:2013-02-06

Abstract: A high temperature-resistant bacteria strain P98-18A1 capable of growing in petroleum hydrocarbons at 55℃ was isolated fromhot produced fluid from Zhongyuan oilfield. This strain was identified as Bacillus sp. based on phenotypic, physiological, biochemical and 16SrDNA sequence analysis. With crude oil and several alkanes degradation experiments, results showed that this strain can effectively utilize C6-C16 n-alkanes, and selectively utilize of the chain longer than C16 alkanes to some extent under the optimum conditions.