Biotechnology Bulletin ›› 2012, Vol. 0 ›› Issue (12): 76-81.

• Study Report • Previous Articles     Next Articles

Analysis of Single-copy Nuclear TPI Gene Sequences of Oryza L. and ItsApplication in Identification of Oryza granulata

Song Yun1, Chen Yan1, Xu Han1, Li Mingfu1, Liu Shiqin2   

  1. 1Institute of Plant Quarantine,Chinese Academy of Inspection and Quarantine,Beijing 100029 ;2Kunming Institute of Botany,ChineseAcademy of Sciences,Kunming 650204
  • Received:2012-05-01 Revised:2013-01-25 Online:2012-12-26 Published:2013-02-06

Abstract: In this paper, single-copy nuclear TPI genes of Oryza L. and its related genera were amplified and sequenced, we analyzedtheir phylogenetic relationships and sequence differences, designed specific primers for Oryza granulata. Sequences analysis indicate thatthere were few variations between cultivar rice, but many variations between wild rice species, especially sharp variations were found for Oryzagranulata. On the basis of these variations, we designed Oryza granulata specified primers for species identification.