Establishment of Effective Regeneration System of Brassica napus L.in vitro

Abstract

Abstract: We examined the effect of the seedling age of sterile seedling, the days and concentrations of growth regulators in precultivation,the concentrations of 6-BA and NAA on rape tissue culture with HC8(Brassica napus L.). We established the technical systemof regeneration of HC8 in vitro with high frequency. The results showed that the best seedling age and the optimal day of pre-cultivation wasfive days for rape tissue culture with HC8, and the optimal concentration of 2, 4-D was 1.0 mg/L. The best medium for callus induction anddifferentiation of cotyledons were MS+2.0 mg/L 6-BA+0.05 mg/L NAA+3.5 mg/L AgNO3 or MS+3.0 mg/L 6-BA+0.1 mg/L NAA+3.5 mg/L AgNO3.With the medium, the ratios of the highest level of callus induction, regeneration and differentiation were 100%, 88% and 108.33%, respectively.The best medium for callus induction and differentiation of hypocotyls were MS+4.0 mg/L 6-BA+0.05 mg/L NAA+3.5 mg/L AgNO3. With themedium, the ratios of the highest level of callus induction, regeneration and differentiation were 95.24%, 81.82% and 104.55%, respectively.The best medium for rooting of regenerated plant was MS basal medium supplemented with 0.5 mg/L NAA with the ratio of the highest level ofrooting(90%).

Key words: Brassica napus L. , Callus induction , Regeneration in vitro , Shoots differentiation

Yang Changyou, Yuan Zhonghou, Zheng Xiaomin, Zhang Tao. Establishment of Effective Regeneration System of Brassica napus L.in vitro[J]. Biotechnology Bulletin, 2013, 0(1): 111-115.

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