Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (1): 78-82.

• Research report • Previous Articles     Next Articles

ZjAPX Gene Improving Resistance to NaCl and Drought Stress in Arabidopsis

Huina1,2,Meng Yuping2 ,Hao Ziqi1,2,Li Qian1,2,Cao Qiufen1,2,3   

  1. 1. College of Biological Engineering，Shanxi University，Taiyuan 030031;
    2. Biotechnology Research Center，Shanxi Academy of Agricultural Sciences，Taiyuan 030031;
    3. Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau，Ministry of Agriculture，P.R.China，Taiyuan 030031
  • Received:2012-07-03 Revised:2013-01-31 Online:2013-01-30 Published:2013-01-30

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Abstract: In order to investigate the function of ZjAPX(Zizyphus jujuba ascorbate peroxidase )in response of plant to osmotic stress.In this study, ZjAPX gene was introduced into Arabidopsis. Wild type(WT)and transgenic type of Arabidopsis were used to the NaCl osmoticstress and drought stress. These results suggested that transgenic seeds germination and seedlings growth were both better than the wild-type.At 10 days to be treated with NaCl osmotic stress and drought stress, real-time quantitative PCR detection showed that the transcription level ofZjAPX in transgenic plants was significantly higher than wide type. These results suggest that high expression of ZjAPX increased plant NaClosmotic stress and drought tolerance.

Key words: ZjAPX , Transgenic , Arabidopsis , NaCl osmotic stress , Drought stress