The Determination of the Taxonomic Status of Two Soil Rhizobia from Southern Tibet

Abstract

Abstract: Polyphasic taxonomy was adopted to determine the taxonomic status of two soil rhizobia from southern Tibet. Two Gramnegative, aerobic, rod-shaped bacteria, designated strains CNU8561007 and CNU85000012. The morphological studies demonstrated that the two test strains had similar features. The slight features differences between CNU8561007 and CNU85000012 might be related to the fact that they were isolated from different geographic regions. According to comparison of 16S rRNA gene sequences, strains CNU8561007 and CNU85000012 were identical and their closest phylogenetic relatives were Rhizobum yanglingense, R.loessense, R.mongolense and R.gallicum. Phylogenetic analysis based on four housekeeping genes（recA, atpD, glnII, and danK）which were in broad agreement with 16S rRNA gene sequence similarities indicated that the two isolates were closely related to Rhizobum yanglingense. The DNA G + C mol% and DNA -DNA hybridization provided supporting evidence that the two test strains belongs to the Rhizobum yanglingense and the BOX-PCR fingerprint profiles demonstrating that the two isolates were not clones of one strain.

Key words: Rhizobia 16S rDNA, Housekeeping genes, DNA-DNA hybridization, BOX-PCR

Shan Huihui, Li Zheng, Han Suzhen . The Determination of the Taxonomic Status of Two Soil Rhizobia from Southern Tibet[J]. Biotechnology Bulletin, 2013, 0(4): 158-166.

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