Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (4): 215-220.

• Research Report • Previous Articles     Next Articles

Comparative Study Based on the LAPS System of Protein Chip Surface Modification Technology

Zhou Shuang1, Jia Yunfang2, Sun Xiaoxuan1, Xing Keli1   

  1. 1. Medical University of Tianjin School of Biomedical Engineering, Nankai University, Tianjin 300070 ； 2. College of Information Technical Science, Tianjin 300071
  • Received:2012-10-18 Revised:2013-04-22 Online:2013-04-22 Published:2013-04-22

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Abstract: The surface of chips were modified by 3 - aminopropyl - trimethoxysilane（（3-aminopropy1）trimethyoxysilane APTES）and glutaraldehyde（Glutaraldehyde, GA）, levodopa（L-DOPA）modification of the chip carrier surface. The two kinds of different types of modification of the protein chips prepared a comparative study. XPS detect surface modification effect of the activator. AFP antibodies were fixed on the modified film base, then adding AFP solution, and photocurrent detected by LPAS system, selecting reactive protein probes as monitoring indicators. The results showed the silicon protein modified by levodopa fixing preferably, having a higher reaction activity and a large detection limit, though the amplitudes were small.

Key words: LAPS, Protein chip, Surface modification