Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (4): 85-89.

• Research Report • Previous Articles     Next Articles

Construction of a Genomic DNA Library of Chlamydomonas reinhardtii

Wang Chaogang1, Chen Aina1, Hu Zhangli1, Yu Shanshan2   

  1. 1. Shenzhen Key Laboratory of Marine Biological Resources and Ecological Environment，College of Life Sciences，Shenzhen University， Shenzhen 518060 ；2. The High School Attached to Shenzhen University Normal College，Shenzhen 518054
  • Received:2012-10-12 Revised:2013-04-22 Online:2013-04-22 Published:2013-04-22

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Abstract: The genomic DNA isolated from Chlamydomonas reinhardtii CC-849 was digested with BamH I and Bgl II. The 6-12 kb genomic DNA fragments were recovered from agarose gel and concentrated to 200 ng/μL. Then they were inserted into λDNA ZAP expression vector, packed by packaging extracts of λ phage and transformed into Escherichia coli XL1-Blue. Finally, the genomic DNA library was obtained with an average insert size of 9 kb. Results showed that the titer of genomic library was 2.12×105 pfu/mL and contained 4.26×104 clones. After amplified, the titer of genomic library was up to 9.5×106 pfu/mL.

Key words: Chlamydomonas reinhardtii, Genomic DNA library, λDNA, ZAP expression vector