Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (6): 116-121.

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Recombinant and Expression of Amidase from Nocardia farcinica in E. coli and Application on PA Modification

Guo Yingchun1, 2 Chen Sheng1 Su Lingqia1 Wu Jing1   

  1. (1. State Key Laboratory of Food Science and Technology, Industrial Biotechnology Key Laboratory of the Ministry of Education, Jiangnan University, Wuxi 214122;2. Institute of Biological Engineering, Jiangnan University, Wuxi 214122)
  • Received:2013-06-20 Revised:2013-06-20 Online:2013-06-20 Published:2013-06-20

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Abstract: The amidase from Nocardia farcinica was recombined and expressed in E. coli. The recombinant vector pET24a/AMID was constructed and transferred into E. coli Origami(DE3). After 20 h induction(0.4 mmol/L IPTG, 25℃), the amidase activity was reached about 1.02 U/mL. In this study, the effect of temperature, pH, treatment time and rotation rate was investigated when PA fabric was treated with recombinant amidase, the water absorbability and wettability was detected by the methods of capillary effect and dying test. The result was demonstrated that the optimization of treatment condition were as follow: 40℃, pH7.5, 100 r/min rotation rate and treated 1 h, under this condition, the rising height of PA was increased by 8.7 cm, the dying percent was increased by 23.1%. The effect of enzymatic modification by recombinant amidase was almost the same as alkali treatment.

Key words: Amidase, Nocaridia farcinica, Recombinant and expression, E. coli, PA modification