Rapid Determination of Diarrhetic Shellfish Poisoning Toxins in Shellfish Samples by Immune Colloidal Gold Strip

Abstract

Abstract: In the present study we determined the Okadaic Acid(OA), the main pathotoxin of diarrhetic shellfish poisoning toxins(DSPs), in shellfish samples in Shanghai sea food markets by the immune colloidal gold strip for the first time which is researched and developed by our lab. There are 69 samples which were collected from May 2011 to October 2011 for each month from the seafood market. The samples were tested by OA immune colloidal gold strip and enzyme-linked immunosorbnent assay(ELISA)after a series of processing. The result showed that there are 11 positive samples contain OA for OA Immune colloidal gold strip, and the positive rate is 15.9％. The 11 positive shellfish samples were Mytilus edulis(n=5), Argopecten irradians(n=4), Ostrea(n=1)and Bullacta exarata(Philippi)(n=1). The result of ELISA showed that the 11 samples which were tested positive by immune colloidal gold strip contain the OA, and the concentration of OA was quantified higher than 15 μg/100 g. The correlation between OA concentration determined via Immune colloidal gold strip and ELISA is 100%. The time of determining the shellfish samples using the OA immune colloidal gold strip is within 30 min from collecting the samples to get the result, and the result is accurate with no false positives. In the present study, immune colloidal gold strips test were shown to be an effective tool for rapid screening in marine environmental monitoring and seafood quarantine and inspection with the limit of detection meeting the safe threshold of OA in shellfish.

Key words: Diarrhetic shellfish poisoning, Okadaic acid, Immune colloidal gold strip, Rapid determination

Zhang Yang, Jia Rui, He Peimin . Rapid Determination of Diarrhetic Shellfish Poisoning Toxins in Shellfish Samples by Immune Colloidal Gold Strip[J]. Biotechnology Bulletin, 2013, 0(6): 221-225.

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