Abstract: In order to identify apple ring rot and make sure of the present situation of apple ring rot’s main pathogenic genes, taking the method of PCR to amplificate and sequence the ITS genes, EF-1α gene and β-tubulin gene. The results showed that the fungi of apple ring rot is Botryosphaeria dothidea. There were EF-1α gene and β-tubulin gene in the apple ring rot’s DNA. Tubulin and transcription factors may be involved in the disease process of apple ring rot, and they are important in the pathogenic process of the apple ring rot.

Key words: Botryosphaeria dothidea, PCR ITS, EF-1α β-tubulin