Cloning and Expression of Helicobacter pylori Urease Subunit A and Its Immune Cross Protection Between Animal Antiserum and Patient Serum

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (8): 135-138.

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Cloning and Expression of Helicobacter pylori Urease Subunit A and Its Immune Cross Protection Between Animal Antiserum and Patient Serum

Liu Wen¹, Tang Yanchao¹, Huang Rui², Cui Haiyan¹, Yuan Jinshan¹, Hu Wei¹

（1. School of Life Sciences，Shandong University of Technology，Zibo 255049；2. School of Basic Medicine，Henan College of Traditional Chinese Medicine, Zhengzhou 450008）

Received:2013-01-30 Revised:2013-08-11 Online:2013-08-11 Published:2013-09-02

Abstract

Abstract: In this paper, immune cross protection from ureA antigen between rabbit antiserum and patient serum, infected by H. pylori, was tested. The ureA gene from H. pylori 26695 strain was amplified with PCR technology and cloned into expressive vector pBVIL1 and the recombinant pBVIL1-ureA was transformed into DH5α strain. The ureA antigen purified was used to immune rabbit three times and antiserum was acquired. The antiserum titre and immune cross protection were tested with ELISA. The results showed that the antigen expressed by prokaryotic system could specifically react on both patient serum and rabbit antiserum and possess a competitive immune cross reaction. As a result, it was concluded that animal antibodies against H. pylori may be applied in immune precaution and therapy of infections diseases from H. pylori.

Key words: Helicobacter pylori, Urease subunit A, Antiserum, Immune protection

Liu Wen, Tang Yanchao, Huang Rui, Cui Haiyan, Yuan Jinshan, Hu Wei. Cloning and Expression of Helicobacter pylori Urease Subunit A and Its Immune Cross Protection Between Animal Antiserum and Patient Serum[J]. Biotechnology Bulletin, 2013, 0(8): 135-138.

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Cloning and Expression of Helicobacter pylori Urease Subunit A and Its Immune Cross Protection Between Animal Antiserum and Patient Serum

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