Identification of Rodents by DNA Barcoding in Inner Mongolia Area

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (8): 94-98.

• Research Report • Previous Articles Next Articles

Identification of Rodents by DNA Barcoding in Inner Mongolia Area

Chang Zili, Liu Fang, Wang Jianjun, Li Jianyun, Hu Yanhong, Wu Zhenghua, Wang Haohui, Li Ke, Yang Zhixian, Ma Zhidong, Fan Mengguang, Zhang Zhongbing

（Inner Mongolia Center of Endemic Disease Control and Research，Hohhot 010031）

Received:2013-03-04 Revised:2013-08-11 Online:2013-08-11 Published:2013-09-02

Abstract

Abstract: To study the feasibility of DNA barcoding in the identification of rodents. 59 specimens of 7 species were sequenced（barcoded）for a 657 bp region of the mitochondrial cytochrome oxidase subunit I gene（COI）in Inner Mongolia Area, China. The GC content and genetic distance was calculated using MEGA5.0, and the phylogenetic trees were constructed with Neighbor-Joining（NJ）method. The results showed that AT contents was larger in all sequences, mostly species had specified amino acid component. The average intraspecific genetic distance（K2P）was 0.6% and the average interspecific genetic distance was 23.1%. A Neighbor-Joining tree showed 7 major clusters apparent. We conclude that COI sequencing of DNA barcoding can be used to identify rodents species in Inner Mongolia.

Key words: DNA barcoding, Rodent, COI gene

Chang Zili, Liu Fang, Wang Jianjun, Li Jianyun, Hu Yanhong, Wu Zhenghua, Wang Haohui, Li Ke, Yang Zhixian, Ma Zhidong, Fan Mengguang, Zhang Zhongbing . Identification of Rodents by DNA Barcoding in Inner Mongolia Area[J]. Biotechnology Bulletin, 2013, 0(8): 94-98.

References

[1] Hebert PDN, Cywinska A, Ball SL, Dewaard JR. Biological identifica-tions through DNA barcodes[J]. Proc Biol Sci B, 2003, 270：313321.
[2] Hebert PDN, Penton EH, Burns JM, et al. Ten species in one：DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator[J]. Proc Natl Acad Sci USA, 2004, 101（41）：14812-14817.
[3] Hebert PDN, Ratnasingham S, deWaard JR. Barcoding animal life cytochrome c oxidase subunit 1 divergences among closely related species[J]. Proc Biol Sci, 2003, 270（suppl. 1）：S96-S99.
[4] Hebert PDN, Stoeckle MY, Zemlak TS. Identification of birds through DNA barcodes[J]. PLoS Biol, 2004, 2（10）：1657-1663.
[5] Ward RD, Zemlak TS, Innes BH. DNA barcoding Australia's fish species[J]. Philos Trans R Soc Lond B Biol Sci, 2005, 360（1462）：1-11.
[6] Hajibabaei M, Singer GAC, Hickey DA. Benchmarking DNA barcodes an assessment using available primate sequences[J]. Genome, 2006, 49：851-854.
[7] Borisenko AV, Lim BK, Ivanova NV. DNA barcoding in surveys of small mammal communities：a field study in Suriname[J]. Mol Ecol Resources, 2008, 8：471-479.
[8] Ivanova NV, Zemlak TS, Hanner RH. Universal primer cocktails for fish DNA barcoding[J]. Molecular Ecology Notes, 2007, 7（4）：544-548.
[9] Kimura. A Simple Method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences[J]. J Mol Evol, 1980, 16：111-120.
[10] Saitou N, Nei M. The neighbor-joining method：a new method for reconstructing phylogenetic trees[J]. Mol Biol Evol, 1987, 4（4）：406-425.
[11] 潘程莹, 胡婧, 张霞. 斑腿蝗科Catantopidae七种蝗虫线粒体COI基因的DNA条形码研究[J]. 昆虫分类学报, 2006, 28（2）：103-110.
[12] 鲁再丰, 蒋国红, 白素英. 亚洲黑熊COI序列变异及其DNA条码的应用[J]. 东北林业大学学报, 2010, 38（10）：79-81.
[13] 严洁, 马晓燕, 杜婕. 中国壁虎属9物种的DNA条码技术[J]. 南京师大学报：自然科学版, 2010, 33（4）：84-90.
[14] Zhang QY, Cheng QQ, Guan WB. Mitochondrial COI gene seque-nce variation and taxonomic status of three macrobrachium species[J]. 动物学研究, 2009, 30（6）：613-619.
[15] 林琪, 李少菁, 黎中宝. 中国东南沿海青蟹属不同种类的mtDNA COI基因序列分析及其系统发育[J]. 厦门大学学报：自然科学版, 2008, 47（2）：268-273.
[16] Agrizzi J, Carolina Loss A, Farro PCA. Molecular diagnosis of Atlantic Forest mammals using mitochondrial DNA sequences Didelphid marsupials[J]. The Open Zoology Journal, 2012, 5, （Suppl. 1-M2）：2-9.
[17] Nicolas V, Schaeffer B, Missoup AD, et al. Assessment of three mitochondrial genes（16S, Cytb, CO1）for identifying species in the Praomyini tribe（Rodentia Muridae）[J]. PLoS One, 2012, 7（5）：e36586.
[18] 马英, 李海龙, 鲁亮. DNA条形码技术在青海海东地区小型兽类鉴定中的应用[J]. 生物多样性, 2012, 20（2）：193-198.
[19] Eaton MJ, Meyers GL, Kolokotronis SO. Barcoding bushmeat：molecular identification of Central African and South American harvested vertebrates[J]. Conserv Genet, 2009, 9：1-16.

Identification of Rodents by DNA Barcoding in Inner Mongolia Area

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments

[1]	Zhou Zhijun, Shang Na, Chang Yanlin, Shi Fuming. DNA Barcodes Reveal Intra-and Inter-Individual Sequence Variation in the Bush Cricket Mecopoda niponensis(Orthoptera：Tettigoniidae) [J]. Biotechnology Bulletin, 2014, 0(5): 129-136.
[2]	Guo Fengjuan, Yang Jianchun, Hu Shijia, Li Yongshi. Identification of Crocodile Production Through DNA Barcode [J]. Biotechnology Bulletin, 2013, 0(8): 99-104.