Abstract:

The best ethanol extraction technology of paclitaxel from leaves of Taxus chinensis var. mairei was established. By a serials of single-factor experiments and L16（43）orthographic experiments, the extraction conditions were determined .The main factors contained Zerkleinerungsgrad 、ethanol concentration 、solid-liquid ratio and extraction time, the results showed that the best extraction conditions as follow ：120 mesh, ethanol concentration was 95%, solid-liquid ratio was 1∶15, the extraction time was 4 hour, repeated 2 times, the leaching temperature was 40℃. Then elutioned with ethyl acetate-acetone（6∶4）through a small chromatographic column, and detected by HPLC.
Under these conditions, the total paclitaxel extraction yield can reach up to 0.012 5 mg/g（dry weight）.

Key words: Taxus chinensis var. mairei, Paclitaxel, Ethanol extraction, HPLC