The Correlation Between Soluble Carbohydrate Metabolism and Lipid Accumulation in Castor Seeds

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.017

Abstract

Abstract: The relationship between soluble carbohydrates metabolism and lipid accumulation in the development of castor seeds was studied by HPLC，RNA-seq sequencing and radioactive carbon isotope tracing method. Soluble carbohydrates in developing castor seeds were mainly composed of glucose，fructose and sucrose，and decreased obviously with seed development and oil accumulation. The significant negative correlation between sucrose content and lipid accumulation(r=0.980)was observed. Hexose-to-sucrose ratio was much higher and the genes related to carbohydrates metabolism were highly expressed in the early stage of seed development. Especially，sucrose synthase played the key role in carbohydrates metabolism. Hexose-to-sucrose ratio was decreased and the genes involved in carbohydrates metabolism were down regulated with the rapid accumulation of seed oil during the middle and late developmental stages，while the expressions of genes related to fatty acid synthesis and lipid accumulation increased significantly. Confirmed by ¹⁴C-sucrose isotope tracing experiments，the conversion of carbohydrates to oil was significantly inhibited by reducing the sucrose intake；consequently lipid accumulation was limited in developing castor seeds. Therefore，soluble carbohydrates metabolism(mainly sucrose)may play an important role in the process of lipid accumulation in castor seeds.

Key words: castor bean, soluble carbohydrates, oil, sucrose synthase, HPLC

ZHANG Yang, LIU Ai-zhong. The Correlation Between Soluble Carbohydrate Metabolism and Lipid Accumulation in Castor Seeds[J]. Biotechnology Bulletin, 2016, 32(6): 120-129.

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