Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (2): 136-142.

• Papers • Previous Articles     Next Articles

Construction of a Phage-displayed Anti-clenbuterol Antibody Library and Selection of Monoclonal Antibody

Dong Jinhua1, Tang Yuhai1, Qiao Ning1, Han Jinhong1, Dong Meihua1, Dong Yiyang2,   

  1. (1. Department of Research and Development,Weifang University of Science and Technology,Shandong 262700;2. College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029)
  • Received:2013-08-12 Online:2014-02-27 Published:2014-02-27

Abstract: An anti-clenbuterol antibody was developed by immunization of mouse and construction of a phage displaying antibody library. The antibody has a novel sequence and the utilization of gene segments in germline was also identified. With this antibody, a competitive assay was performed and gave a limit of detection of 15.6 ng/mL. The IC50 of phage-antibody with free clenbuterol against immobilized bovine serum albumin conjugated clenbuterol was 0.602 μg/mL. All the results suggested that the antibody is useful in practical applications.