The Isolation，Screening and Identification of CaCO3 Mineralization Strain from Sand Boon

Abstract

Abstract: Sand bone（named by the research team）is a tube that is agglutinated by sand with cementing material mainly composed of calcium carbonate. It was found in Zhongwei area of Ningxia Autonomous Region. Strain 3# has strong ability of CaCO₃ mineralization among 12 strains isolated from sand bone. Mineral deposit was produced and covered the flask wall after 72 h shake cultivation with 1.0% CaCl₂ liquid medium. To analyze the mineral deposits, XRD and SEM were performed. The result was showed that mineral deposit was made up of calcium carbonate, which contains two crystal forms（calcite and vaterite）. Based on morphological, physiological and biochemical properties and 16S rDNA sequence analysis, strain 3# was identified as Paenibacillus sp., and was named Paenibacillus sp. s3 . It laid a good foundation for reaching formation mechanism of sand boon further.

Hu Weilian, Dai Dehui. The Isolation，Screening and Identification of CaCO₃ Mineralization Strain from Sand Boon[J]. Biotechnology Bulletin, 2014, 0(2): 171-175.

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The Isolation，Screening and Identification of CaCO₃ Mineralization Strain from Sand Boon

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