Progress in the Research Regarding to the Function of Protein Rep78/68

Abstract

Abstract: Rep78/68, a type of nonstructural proteins encoded by adeno-associated virus（AAV）genome, have a variety of control functions. Rep78/68 promote the integration of AAV DNA site-specific into human chromosome 19, suppress the virus biological activity, such as adenovirus, simian virus 40, adjust the AAV DNA replication and transcription, etc. This paper mainly discusses the mechanism of site-specific integration, the mechanism of Rep78 / AAV ITR/ICP8 complex formation and the characteristics of the oligomerization of Rep68 protein, the limit of the nuclear output of p53 by Rep78 protein, the interaction between AAV Rep78 and human papilloma virus（HPV）E1 protein prompt the combination of Rep78 and ITR DNA, inhibition of the hepatitis B virus（HBV）by Rep78. While the detailed mechanism still remaines to be improved and enriched, further elucidatation of Rep78/68 protein related mechanism may improve the production technology of rAAV and develop gene drug based on rAAV, which has a great significance.

Zhan Shenbiao, Lü Yinghui, Li Zhaofa,. Progress in the Research Regarding to the Function of Protein Rep78/68[J]. Biotechnology Bulletin, 2014, 0(2): 24-29.

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Progress in the Research Regarding to the Function of Protein Rep78/68

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