Application of Molecular Biological Methods to Research on the Genetic Diversities of Virioplankton

Abstract

Abstract: Viruses are recognized as an important component of aquatic microbial communities in marine and freshwater environments, and play a significant role in regulating microbial loop, driving global biogeochemical cycling and maintaining clonal diversities of phytoplankton and bacterioplankton populations. However, the structure and diversity of virioplankton communities have not been extensively and overall investigated using classical viral culture and quantitative methods, and the rapid development and application of molecular-ecological techniques offer a new way. The basic concept of some molecular biological methods including cloning library, PFGE, DNA fingerprinting, DNA chip and metagonemics were described, and their application to address the population composition and genetic diversities of virioplankton in natural aquatic environment, and to investigate the ecological relationship between virioplankton and environmental factors.

Gao Ebin, Wang Ziqian. Application of Molecular Biological Methods to Research on the Genetic Diversities of Virioplankton[J]. Biotechnology Bulletin, 2014, 0(2): 47-55.

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