Abstract: The purity identification of Reyan No.2 was performed by using SSR marker technique. The results showed that 5 SSR markers were polymorphic between the parents of Reyan No.2, among which 4 were co-dominant markers, one was dominant marker. To ensure the accuracy and reliability, 4 co-dominant SSR markers were used for purity identification of Reyan No.2. The hybrid purity was 98.82%, which was completely consistent with field identification. It is feasible to conduct purity identification of Reyan No.2 using SSR markers.