Isolation and Identification of an Actinomycetes Strain Against Root-knot Nematode from Mangrove Soil

Abstract

Abstract: 110 Actinomycetes strains were isolated by plate dilution method and anti-root-knot nematode strain HA10401 was screened by 24-well plate fluid model from mangrove soil in Dongzhaigang National Nature Reserve, Hainan Province. While the fermentation broth was diluted by 20 times and 40 times, the mortality rates against nematode were 58.2% and 52.6%, respectively. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain HA10401 was closely related to Streptomyces variabilis with the highest similarity of 99.8%, and the two strains formed a cluster in the phylogenetic tree. The morphological, cultural and biochemical characteristics were basically accorded with Streptomyces variabilis. As a result, strain HA10401 was identified as Streptomyces variabilis, and its anti-root-knot nematode activity was first reported in this study and worth further research.

Key words: Mangrove, Actinomycetes, Identification, Root-knot nematode

Chen Yuqing, Huang Huiqin, Liu Min, Bao, Shixiang. Isolation and Identification of an Actinomycetes Strain Against Root-knot Nematode from Mangrove Soil[J]. Biotechnology Bulletin, 2014, 0(10): 174-178.

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