Cloning and Expression Analysis of Sugarcane Isoflavone Reductase-like(IRL)Gene

Abstract

Abstract: The SoIRL gene cDNA sequence was cloned from sugarcane variety GT11 using RT-PCR techniques. The bioinformatics methods were used to analyze the putative amino acid sequence, and Real-time PCR method was used to study the expression of SoIRL gene in different tissues and under different stresses. The results showed that the full-length cDNA of SoIRL（GenBank accession number：KF808324）in sugarcane was cloned. The sequence consists of 1 167 bp with an intact open reading frame of 927 bp, encoding a polypeptide of 303 amino acids. Phylogenetic tree analysis indicated that SoIRL was highlg closely related to IRL of Zea mays. Real-time PCR results showed that the SoIRL expressed in root, stalk and leaf. Furthermore, SoIRL transcription level was induced under the treatment of the bacterial of ratoon stuning diaease, low temperature, PEG, NaCl and ABA stresses, but the expression patterns were different. Gene SoIRL was firstly isolated and characterized from sugarcane（GT11）, which may participate in sugarcane resistance to RSD, and also play a role in the sugarcane resistance to chilling, drought and salt stress environments.

Key words: Sugarcane, SoIRL , Gene cloning, Expression analysis

Xie Xiaona,Zhang Xiaoqiu,Shao Min,Zhu Hui,Yang Litao,Li Yangrui. Cloning and Expression Analysis of Sugarcane Isoflavone Reductase-like(IRL)Gene[J]. Biotechnology Bulletin, 2014, 0(11): 130-137.

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