Abstract: In order to provide a foundation for the application of the Trichoderma reesei FS10-C on remediation of heavy metals-contaminated soils, the solid fermentation substrate and the fermentation conditions were studied. The fermentation substrate was screened through a single factor, then confirmed the appropriate fermentation conditions using a method of orthogonal combination and different factors. In addition, an enlarge experiment was also carried out in a 50 L solid fermentation tank. The result showed that the mixture of orange peel and wheat bran were deemed the best fermentation material for Trichodermaressei FS10-C. The optimal conditions in plate as following:the proportion of orange peel to bran and solid material to water were 1∶1 and 1∶1.2 respectively, inoculum 5%, the temperature of fermentation was 28℃. Under this optimal fermentation conditions, the spore number were up to 2.43×10 ¹⁰ CFU/g after 14 days, and the effect was satisfactory when it had been applied to solid fermentation tank, the spore number were 1.44×10¹⁰ CFU/g. Through the screening of soild media and optimization of the fermentation conditions, the cost of production was reduced, and FS10-C biomass through the optimization of fermentation conditions was increased. The solid fermentation of FS10-C had the potential of mass production.

Key words: Trichoderma reesei,FS10-C,Solid fermentation,Fermentation conditions, Bioremediating inoculant