Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (3): 106-110.

• Papers • Previous Articles     Next Articles

Isolation and Identification of Cellulose-Decomposing Microorganisms in Deep-litter System

Liao Qing1, Jiang Zhepu1, Xingying1, Wei Guangpo1, Huang Dongliang2,3, Li Yangrui3   

  1. (1. Agricultural Resources and Environment Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007;2.Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007;3. Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007)
  • Received:2013-10-22 Online:2014-03-29 Published:2014-03-31

Abstract: 43 cellulose-decomposing microorganisms were isolated from deep-litter systems. Five of them, with bigger transparent circles and disintegration effect of filter paper piece were screened using Congo red differential medium and filter paper method. Then 2 strains with higher capacity of decomposing cellulose were obtained by determinations of CMC enzyme activity, FPA enzyme activity and natural cellulase activity, and named as F5 and F21, respectively. The 2 strains were identifined as Bacillus subtilis and Streptomyces sp. basing on 16S rDNA phylogenetic analysis.

Key words: Deep-litter system, Cellulose-decomposing microorganisms, Screening, 16S, rDNA, Identification