Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (5): 148-154.

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The Discovery and Identification of Yeast Can Produce Cutinase, and Optimization of Fermentation Conditions

Ran Qinqin1,2 Zhang Xiaoning2 Zhang Wenkun1 Zhang Xuejun1,2 Zhang Mengmeng1   

  1. (1. Guizhou Province Key Laboratory of Fermentation Engineering & Biological Pharmacy, Guizhou University, Guiyang 550025;2. School of Brewing and Food Engineering,Guizhou University,Guiyang 550025)
  • Received:2013-12-03 Online:2014-05-23 Published:2014-05-24

Abstract: To get effective enzyme hydrolyze cutin of Eucommia ulmoides corneous layer, the extraction of the biological active compositions within Eucommia ulmoides was done using enzymatic hydrolysis. This article from the lesion Eucommia leaves isolated bacteria capable of producing cutinase and identify the bacteria, confirmed that the strain belongs to Rhodotorula mucilaginosa, and the strain producing cutinase fermentation conditions were optimized. This finding is significant, because the yeast more secure than plant pathogens is more suitable for mass production.

Key words: Eucommia ulmoides, Cutinase Isolation, Identification, Rhodotorula, Nucilaginosa