Study on Purification of Cellulase from Aspergillus niger AS0006

Abstract

Abstract: Two endoglucanases（EG）components and a cellobiohydrolase（CBH）from Aspergillus niger AS0006 were separated and purified by（NH₄）₂SO₄ fractional precipitation, Sephadex G-25 and Sephadex G-100 chromatography. According to cellulase activity and electrophoresis detection found that the molecular weight of two EG components were 29.63 kD and 37.49 kD, respectively, the molecular weight of CBH component was 56.51 kD. Compared to the crude enzyme, the recovery rate of EG components could reach 18.07%, the purification fold was 3.46, the recovery rate of CBH component could reach 12.96%, the purification fold was 4.14, the recovery rate of β-glucosidase（CB）could reach 22.37% and the purification fold was 3.58.

Key words: Aspergillus niger, Cellulase, Purification

Zhang Huan,Cao Yanxin,Jiang Lin,Wang Xiaoming,Liu Qi,Dong Xiaoying,Kou Wei. Study on Purification of Cellulase from Aspergillus niger AS0006[J]. Biotechnology Bulletin, 2014, 0(6): 187-191.

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