Establishment and Application of a Specific PCR Assay for Rapid Identifying Thermoactinomyces intermedius

Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (7): 60-63.

• Technique • Previous Articles Next Articles

Establishment and Application of a Specific PCR Assay for Rapid Identifying Thermoactinomyces intermedius

Zhang Mingjuan¹, Yao Su¹, Li Hui¹, Liu Yang¹, Xin Chunhui², Xu Ling², Cheng Chi¹

1. Chinese Food Fermentation Industry Research Institute Chinese industrial culture collection center, Beijing 100015；
2. Shandong Bandaojing Limited by Share Ltd, Gaoqing 256300

Received:2014-01-03 Online:2014-07-15 Published:2014-07-16

Abstract

Abstract: This study establishs a rapid specific PCR assay to screen Thermoactinomyces intermedius strains and screens Thermoactino-myces intermedius strains from high-temperature daqu of sesame flavor liquor by the specific PCR assay. Experimental results show that the designed specific primers are specific for Thermoactinomyces intermedius, it is suitable for screening rapidly Thermoactinomyces intermedius strains. The specific PCR can save a lot of time and costs compared with the traditional identifying methods.

Key words: Specific PCR, Thermoactinomyces intermedius, Rapid identifying

Zhang Mingjuan, Yao Su, Li Hui, Liu Yang, Xin Chunhui, Xu Ling, Cheng Chi. Establishment and Application of a Specific PCR Assay for Rapid Identifying Thermoactinomyces intermedius[J]. Biotechnology Bulletin, 2014, 0(7): 60-63.

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Establishment and Application of a Specific PCR Assay for Rapid Identifying Thermoactinomyces intermedius

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