Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (8): 70-75.

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Comparison of Four Methods for Whole Genomic DNA Extraction from Bemisia tabaci

Dai Tianmei1, Lü Zhichuang1, Wan Fanghao1,2   

  1. 1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193;
    2. Center for Management of Invasive Alien Species Ministry of Agriculture, Beijing 100193
  • Revised:2014-01-24 Online:2014-08-15 Published:2014-08-01
  • Contact: 通讯作者: 万方浩，男，博士，研究员，研究方向：入侵生物学；E-mail：wanfanghao@caas.cn
  • Supported by:
    国家自然科学基金项目(31100269);“973”计划(2009CB119200);公益性行业(农业)科研专项(201303019)

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Abstract: Obtaining high-quality DNA is a prerequisite to study the molecular mechanisms of many organisms. For tiny insects, low DNA concentration（from one single insect）is unable to meet the need of some experiments. To find a convenient and efficient method for whiteflies genome extraction, KAc, salting-out, chloroform-isoamylol and phenol methods were used to extract bull whiteflies genome DNA, respectively. The efficiency of different DNA extraction methods were evaluated by comparing the concentration, purity, PCR products of DNA, extraction time and the toxicity of the reagents, respectively. The results showed that the average concentration of DNA extraction of salting-out method was 521 ng/μL, and the purity could meet the experiment requirements, and the PCR product brand of MSAP primer amplification was bright. Compared to other methods, salting-out method was more simple, rapid and non-toxic. Therefore, salting-out method was the best ideally method to extract the whole genomic DNA from whiteflies.

Key words: DNA extraction, Bemisia tabaci, KAc method, Chloroform-isoamylol method, Salting-out method, Phenol method