Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (2): 217-221.doi: 10.13560/j.cnki.biotech.bull.1985.2015.02.032

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The Influence Factors of Determination of Haematobacter sp. Cellular Fatty Acids

He Weihong1, An Mingli1, Chen Guocan1, Jia Bin1，3, Wu Xiaolei2, Wang Yanan1   

  1. 1. Key Laboratory of Microbial Engineering of Henan Province, Zhengzhou 450008;
    2. College of Engineering, Peking University， Beijing 100871;
    3. College of Life Sciences, Shenzhen University, Shenzhen 518060
  • Received:2014-10-04 Online:2015-02-05 Published:2015-02-06

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Abstract: Cellular fatty acid composition of strain HNMC11807, a novel species belong to Haematobacter sp., were detected by used Sherlock MIS system of MIDI company USA. The influence by different culture methods and cells growth conditions on cells fatty acid composition were explored in this article. Based on the bacterial cells cultured in varied medium such as in different medium components and in solid or liquid medium, the cellular fatty acid composition were respectively analysed and compared. On the same time, two standard systems in this instrument, conventional and fast, were used to run samples, and the detect results of the cellular fatty acid composition were compared respectively. As the results, the cells fatty acid composition was closely related with culture condition and a significant differences. The cellular fatty acid composition must be carried out in specified and unified culture conditions when this system was used in microbial classification.

Key words: determination of cells fatty acids, condition of medicum, standards of fatty acids, Haematobacter sp.