Expression of microRNA-483 and microRNA-486 in the Cloned and fat-1-transgenic Bovine

doi:10.13560/j.cnki.biotech.bull.1985.2016.03.017

Abstract

Abstract: The expression levels of microRNA-483 and microRNA-486 in heart, liver, spleen, lung, kidney, placenta, cotyledons, endometrium from normal, cloned, and transgenic bovine were measured by real time quantitative PCR. The results showed that microRNA-483 and microRNA-486 were expressed in all tissues of normal, cloned and fat-1-transgenic bovine；significantly higher in the heart than other tissues. However, the expression levels of microRNA-483 and microRNA-486 in transgenic bovine were lower than normal one. Further, the expressions of microRNA-483 and microRNA-486 varied in different tissues and high in the heart, indicating that the expressions of microRNA-483 and microRNA-486 may be correlated with pathophysiological process of myocardial hypertrophy and myocardial infarction.

Key words: microRNA-483, microRNA-486, transgenic bovine, heart

Lü Yang, WANG Yu, SUN Jia-jia, GONG Chun-ling, LI Guang-peng. Expression of microRNA-483 and microRNA-486 in the Cloned and fat-1-transgenic Bovine[J]. Biotechnology Bulletin, 2016, 32(3): 105-108.

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