[1]Baeshen NA, Baeshen MN, Sheikh A, et al. Cell factories for insulin production[J]. Microb Cell Fact, 2014, 13:141. [2]Clare JJ, Rayment FB, Ballantine SP, et al. High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene[J]. Biotechnology(N Y), 1991, 9:455-460. [3] 崔蕴霞, 明文玉, 银巍, 等. 人重组白蛋白基因在巴斯德毕赤酵母中的高效表达[J]. 微生物学报, 2001, 41(2):244-247. [4] 马银鹏, 王玉文, 党阿丽, 等. 毕赤酵母表达系统研究进展[J]. 黑龙江科学, 2013, 9:27-31. [5] Kjeldsen T, Brandt J, Andersen AS, et al. A removable spacer peptide in an alpha-factor-leader/insulin precursor fusion protein improves processing and concomitant yield of the insulin precursor in Saccharomyces cerevisiae[J]. Gene, 1996, 170:107-112. [6] Kjeldsen T, Hach M, Balschmidt P, et al. Prepro-leaders lacking N-linked glycosylation for secretory expression in the yeast Saccharomyces cerevisiae[J]. Protein Expr Purif, 1998, 14:309-316. [7] Kjeldsen T, Pettersson AF, Hach M. Secretory expression and characterization of insulin in Pichia pastoris[J]. Biotechnol Appl Biochem, 1999, 29(Pt 1):79-86. [8] Kjeldsen T. Yeast secretory expression of insulin precursors[J]. Appl Microbiol Biotechnol, 2000, 54:277-286. [9] 刘海峰, 周祥山, 庞甲佩, 等. 毕赤酵母表达人胰岛素前体的纯化和N末端不均一性分析[J]. 华东理工大学学报:自然科学版, 2013, 39(5):565-571. [10] 秦秀林, 钱江潮, 储炬. 利用间隔肽促进S-腺苷甲硫氨酸合成酶在毕赤酵母中的分泌表达[J]. 生物技术通报, 2013(6):140-146. [11]熊爱生, 彭日荷, 李贤, 等. 信号肽序列对毕赤酵母表达外源蛋白质的影响[J]. 生物化学与生物物理学报, 2003, 35(2):154-160. [12]何尧声. 重组人胰岛素在毕赤酵母中的分泌表达[D]. 海口:海南大学, 2008. |