Abstract: Selecting the hydrolysate of poplar wood sawdust for fermentation substrate,we used Acetobacter xylinum to prepare bacterial cellulosee,and further studied the effect of reducing sugar concentration,the initial pH,inoculum size,fermentation temperature,and fermentation time on production,water holding capacity,and rehydration rate of bacterial cellulose. The results showed that the production of bacterial cellulose was 3.14 g/L,water holding capacity of bacterial cellulose was 98.72%,and bacterial cellulose rehydration was 86.69% when reducing sugar concentration of hydrolyzed poplar wood sawdust was 2.5%,the initial pH was 6.0,inoculum size was 6%,fermentation time was 6 d,and fermentation temperature was 30℃.The detection results of scanning electron microscope,infrared spectroscopy,and thermo-gravimetric analysis indicated that the structure of synthetic product was super fine mesh and the groups of the product corresponded to the structure of cellulose.

Key words: hydrolysate of poplar wood sawdust, scanning electron microscope, infrared spectroscopy, thermo-gravimetric analysis