Cloning,Expression,and Insecticidal Activities of Gene cry2Ab34 from Bacillus thuringiensis

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.024

Abstract

Abstract: In order to identify and detect insecticidal activities from Bacillus thuringiensis strain,the genomic DNA was extracted from the strain Bt BJH500 with high insecticidal activity,from which the insecticidal gene cry2Ab34（GenBank accession number：KX357382）was cloned by PCR. The gene was designated as cry2Ab34 by International Gene Nomenclature Committee of Bt. Gene cry2Ab34 was expressed and the weight of expressed product was approximate 70 kD. The gene was expressed in Escherichia coli BL21 for the determination of biological activity,results showed that this gene presented certain insecticidal activity to Plutella xyllostella and Helicoverpa armigera of Lepidoptera pests,the corrected mortality of 10 µg/mL was 31.25%,of 100 µg/mL was 62.5 %,of 10 µg/mL was 29.4 %,and of 100 µg/mL was 52.9 %.

Key words: Bacillus thuringiensis, gene cry2Ab34, insecticidal crystal protein, insecticidal activity, Plutella xyllostella, Helicoverpa armigera

ZHANG Yue, LI Hai-tao, LIU Rong-mei, GAO Ji-guo. Cloning,Expression,and Insecticidal Activities of Gene cry2Ab34 from Bacillus thuringiensis[J]. Biotechnology Bulletin, 2017, 33(4): 185-190.

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