iTRAQ-based Quantitative Proteomic Analyses of Differentially Expressed Proteins in Nicotine-induced SH-SY5Y Cells

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.012

Abstract

Abstract: SH-SY5Y,induced by nicotine,was used to seek the effects of nicotine exposure on the alteration of proteins expression profile. The differentially expressed proteins in nicotine-induced neuronal cells were identified using the iTRAQ and nano-LC-Q-TOF MS proteomics,and their bioinformatics was also analyzed. The results showed that the levels of 132 proteins were differentially altered in response to nicotine（| ratio | ≥ 1.5,P<0.05）,including proteins involved in gene expression,MAPK cascade,GTP signal transduction and neurotrophin TRK receptor signaling. Together,our data suggested that the nicotine might elicit dependence by altering the neuronal balance of proteasome subunits,with the MAPK cascade and ubiquitin-proteasome pathway playing a pivotal role.

Key words: differential proteomics, iTRAQ, nicotine, neuronal SH-SY5Y

ZHU Bei-bei, LI Xiang-yu, CHEN Huan, WANG Hong-juan, HOU Hong-wei, HU Qing-yuan. iTRAQ-based Quantitative Proteomic Analyses of Differentially Expressed Proteins in Nicotine-induced SH-SY5Y Cells[J]. Biotechnology Bulletin, 2017, 33(4): 90-97.

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