Cloning,Expression Analysis and Regulation to NF-κB Pathway of Btnl5 in Porcine

doi:10.13560/j.cnki.biotech.bull.1985.2016-1151

Abstract

Abstract:

Porcine butyrophilin-like 5（Btnl5）belongs to butyrophilin family and most of its homologous proteins are immune regulators. In order to explore the sequence characteristics and function of Btnl5,full-length cDNA of Btnl5 was cloned and characterized by reverse transcription PCR and rapid amplification of cDNA ends（RACE）. Then the mRNA expression abundances of Btnl5 in different tissues were detected using quantitative PCR,and its effect on NF-κB pathway was detected by reporter gene assay. The full length cDNA of Btnl5 was 3 334 bp containing a 1 680 bp open reading frame（ORF）encoding 559 amino acids. Structural analysis showed that this protein was a transmembrane protein,and its extracellular region contained a signal peptide,two immunoglobulin domains,its intracellular region was B30.2 domain. Quantitative PCR analysis showed that Btnl5 transcripts almost exclusively expressed in jejunum among 11 detected tissues. Reporter gene analysis indicated that Btnl5 suppressed the NF-κB pathway. The above results suggest that Btnl5 may involve in intestinal immune regulation through inhibiting NF-κB signaling pathway.

Key words: porcine, butyrophilin-like 5, expression analysis, reporter gene assay, nuclear factor κB

BAO Qi, ZHAO Kai, DUAN Zi-yuan. Cloning,Expression Analysis and Regulation to NF-κB Pathway of Btnl5 in Porcine[J]. Biotechnology Bulletin, 2017, 33(6): 142-148.

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