Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (7): 89-95.doi: 10.13560/j.cnki.biotech.bull.1985.2016-1114

• CONTENTS • Previous Articles     Next Articles

Cloning and Expression Analysis of Jc5β-StR Gene in Jatropha curcas

HUANG Yao-yao1, WEN Jin-fen2 ,DENG Ming-hua3, GONG Ming4 ,CHEN Hao-wei1   

  1. 1. Faculty of Modern Agricultural Engineering,Kunming University of Science and Technology,Kunming 650500;
    2. Faculty of Architecture and City Planning,Kunming University of Science and Technology,Kunming 650500;
    3. Faculty of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650201;
    4. School of Life Sciences,Yunnan Normal University,Kunming 650500
  • Received:2016-12-08 Online:2017-07-11 Published:2017-07-11

Abstract: The CDS sequence of Jc5β-StR gene was cloned with Jatropha curcas cDNA as template,and analyzed by bioinformatics analysis. The opening reading frame(ORF)of Jc5β-StR was 1 173 bp encoded 390 amino acids. The predicted molecular weight was 44.23 kD,and theoretical isoelectric point(pI)was 5.22. Blast searching and phylogenetic analysis showed that Jc5β-StR had highest identity(87%)and closest relationships with the 5β-StR of Ricinus communis. Jc5β-StR was a short-chain reductase,containing a progesterone 5β reductase(5β-POR)in a family of short chain reductase(SDRs),and also 6 conserved domains of SDRs. Jc5β-StR expressed in different tissues of root,stem,leave,flower,seed capsule,and seed,the 1highest in seed;moreover,the expression gradually rose with the development of seed,and declined after peak in the later stage of seed development(50 d). The abiotic stress such as NaCl,PEG,4℃,and mechanical damage resulted in the up-regulation of Jc5β-StR expression in varied level,indicating that Jc5β-StR involved in the response to abiotic stress.

Key words: Jatropha curcas L., Jc5β-StR, clone, bioinformatics analysis, expression analysis