Preliminary Study on the Function of Fv-Afe1 Gene in Flammulina velutipes

doi:10.13560/j.cnki.biotech.bull.1985.2018-0019

Abstract

Abstract: According to genome data of a Flammulina velutipes strain L11，we selected Fv-Afe1 belonging to adenylate-forming enzymes superfamily. Transcriptome of F. velutipes was used to analyze gene structure，bioinformatics software to predict the amino acid sequence of Fv-Afe1，the predicted results and amino acid sequences of other species in NCBI were combined to construct phylogenetic tree，and qRT-PCR and DGE（digital gene expression profiling）data to analyze the gene expression in different growth stage. The results showed Fv-Afe1 gene contained 967 bp，including 4 exons and 3 introns. The results of bioinformatics revealed that the gene had two domains at tertiary structure of amino acids. Besides，the product of Fv-Afe1 was intracellular enzyme and no transmembrane structure，Fv-Afe1 belonging to the adenylate-forming enzymes superfamily was confirmed by analyzing phylogenetic tree，expression test showed that Fv-Afe1 had the highest expression in stipe-elongation stage and the secondly highest in stipe-maturation.

Key words: Flammulina velutipes, fruit body forming, adenylate-forming enzymes superfamily

JIANG Si-yuan, CHOU Tian-sheng, LI Xiao, HUANG Rong-mei, XIE Bao-gui. Preliminary Study on the Function of Fv-Afe1 Gene in Flammulina velutipes[J]. Biotechnology Bulletin, 2018, 34(3): 230-234.

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